美洲板栗愈伤组织诱导和植株再生的研究

在培养基中添加不同植物生长调节剂(生长素和细胞机动素),对美洲板粟愈伤组织的诱导以及植株再生进行了研究.将试管繁殖的小植株切为长0.5 cm的小段作为外植体材料,以木本植物培养基加上3%的蔗糖和0.7%的琼脂作为基本培养基,再加上不同浓度的生长素或细胞机动素作为试验处理.生长素包括IBA,NAA,2,4-D和2,4,5-T;细胞机动素包括kinetin,CPPU,thidiazuron(TDZ)和zeatin.结果表明:外植体的植株再生和愈伤组织诱导百分率以及再生植株的形态发生以及愈伤组织的诱导量在不同的植物生长调节剂处理间和同一植物生长调节剂不同浓度的处理间都存在着显著的差异.促使外植体植株再生百分率最高的细胞机动素为TDZ,其次为CPPU,kinitin和zeatin;促使外植体愈伤组织诱导百分率最好的生长素为2,4,5-T,其次为2,4-D,IBA和NAA.

In Vitro Callus Induction and Shoot Initiation of American Chestnut (Castanea dentata)

In vitro callus induction and shoot initiation of the American chestnut(Castanea dentata)were investigated in response to different plant growth regulators(auxins and cytokinins)in the culture medium.Micropropagated shoots were used as explant materials.Auxins included indolebutyric acid(IBA),naphthaleneacetic acid(NAA),2,4-dichlorophenoxyacetic acid(2,4-D),and 2,4,5-trichlorophenoxyacetic acid(2,4,5-T).Cytokinins included kinetin,N-(2-chloro-4-pyridyl)-N'-phenylurea(CPPU),thidiazuron(TDZ),and zeatin.Woody plant medium(WPM)salts containing 3% sucrose and 0.7% agar were used as the basic culture medium.Differences in the number of shoots(primodia),morphology of micropagated shoots,and amount of callus were observed between the plant growth regulator treatments and concentrations.Explants cultured in media containing cytokinins produced more shoots(primodia)with TDZ producing the most,followed by CPPU,kinetin and zeatin.Explants cultured in media containing auxins enhanced callus induction with 2,4,5-T achieving the most,followed by 2,4-D,IBA and NAA.