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杏果实黑斑病菌及近似链格孢种的RAPD分析
引用本文:刘开启,杨炜华,郭泺. 杏果实黑斑病菌及近似链格孢种的RAPD分析[J]. 仲恺农业技术学院学报, 2004, 17(1): 1-6
作者姓名:刘开启  杨炜华  郭泺
作者单位:1. 仲恺农业技术学院植物保护系,广东,广州,510225
2. 山东农业大学植物保护学院,山东,泰安,271018
摘    要:运用随机扩增技术对来源于杏果实的黑斑病菌及其他5个链格孢(Alternaria)相似种共28个菌株的基因组DNA进行了多态性分析,利用10个随机引物获得157条RAPD标记.聚类分析结果表明,9个杏果实黑斑病菌菌株(AX1~AX9)的亲缘关系极为相近,并且是独立于其它供试种群的遗传群体.各供试菌株既具有遗传组成上的相似性,又存在种间及种内遗传分化.

关 键 词:链格孢(Alternaria)  杏果实黑斑病菌  近似种
文章编号:1006-0774(2004)01-0001-06
修稿时间:2003-09-02

RAPD analysis on apricot fruit black spot pathogen and the similar species of Alternaria
LIU Kai-qi,YANG Wei-hua,GUO Luo. RAPD analysis on apricot fruit black spot pathogen and the similar species of Alternaria[J]. Journal of Zhongkai Agrotechnical College, 2004, 17(1): 1-6
Authors:LIU Kai-qi  YANG Wei-hua  GUO Luo
Affiliation:LIU Kai-qi~1,YANG Wei-hua~2,GUO Luo~2
Abstract:Genetic diversities of 28 isolates of apricot fruit black spot pathogen and 5 similar species of Alternaria were analyzed with random amplified polymorphic DNA(RAPD). One hundred and fifty-seven loci were obtained with 10 primers. The results of cluster analysis indicated that 9 isolates (AX1-AX9) of apricot fruit black spot pathogen had very close relationships and could be classified into the same genetic group, which were different from other tested species. The genetic relationships of all the tested isolates were close but there were still considerable genetic diversities.
Keywords:RAPD
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