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鸡肠道菌基因组DNA的RAPD分类的研究
引用本文:吴天星,金勇丰,曹广力,张耀洲. 鸡肠道菌基因组DNA的RAPD分类的研究[J]. 畜牧兽医学报, 2002, 33(3): 247-249
作者姓名:吴天星  金勇丰  曹广力  张耀洲
作者单位:1. 浙江大学理学院,杭州,310027
2. 浙江大学生物化学研究所,杭州,310027
基金项目:浙江省“九五”重点项目资助
摘    要:本文以7种鸡肠道菌为研究对象,分别提取基因组DNA,利用随机扩增多态性DNA(RAPD)技术对其基因组进行DNA分析,筛选出一系列随机引物,能在7种肠道细菌中有较好的多态性,可用作分子标记鉴别这7种肠道菌。

关 键 词:鸡 肠道菌 基因组DNA RAPD分类的研究
文章编号:0366-6964(2002)03-0247-03

Classification of Chicken Intestinal Microflora Species Using RAPD Analysis
WU Tian xing ,JIN Yong feng ,CAO Guang li ,ZHANG Yao zhou. Classification of Chicken Intestinal Microflora Species Using RAPD Analysis[J]. Chinese Journal of Animal and Veterinary Sciences, 2002, 33(3): 247-249
Authors:WU Tian xing   JIN Yong feng   CAO Guang li   ZHANG Yao zhou
Affiliation:WU Tian xing 1,JIN Yong feng 2,CAO Guang li 2,ZHANG Yao zhou 2
Abstract:In this study,a molecular approach based on random amplified polymorphilic DNA has been used for the detection and identification of Peptostreptococcus,Bacteroides,Eubacteri,Bifidobacterium,Bacillus,E.coli and Clostridium,the seven major constituents of chicken intestinal microflora.Some RAPD markers are selected to distinguish among seven intestinal microflora species and shows promise for use in rapid diagnostic tests.
Keywords:Chicken  Intestinal microflora  Classification
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