| 松材线虫rNDA-ITS1区分子检测与鉴定 |
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| 引用本文： | 李一农,余道坚,李芳荣,谢伟宏,陈杰,张青汉. 松材线虫rNDA-ITS1区分子检测与鉴定[J]. 植物保护, 2004, 30(3): 61-63 |
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| 作者姓名： | 李一农 余道坚 李芳荣 谢伟宏 陈杰 张青汉 |
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| 作者单位： | 深圳出入境检验检疫局 深圳 518000 |
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| 摘 要： | 利用两对松材线虫特异性引物，分别对来自日本、美国和葡萄牙的松材线虫虫样进行PCR检测，成功扩增出330bp和220bprDNA-ITS1区基因片段，该方法对松材线虫成虫或幼虫均能作出准确鉴定。
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| 关 键 词： | 松材线虫 rNDA-ITS1 分子检测 PCR检测 鉴定 |
Molecular identification of Bursaphelenchus xylophilus by PCR amplification of first internal transcribed spacer of ribosomal DNA |
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| LI Yi-nong;YU Dao-jian;LI Fang-rong;XIE Wei-hong;CHEN Jie;ZHANG Qing-han. Molecular identification of Bursaphelenchus xylophilus by PCR amplification of first internal transcribed spacer of ribosomal DNA[J]. Plant Protection, 2004, 30(3): 61-63 |
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| Authors: | LI Yi-nong YU Dao-jian LI Fang-rong XIE Wei-hong CHEN Jie ZHANG Qing-han |
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| Affiliation: | Shenzhen Entry-Exit Inspection and Quarantine Bureau;Shenzhen 518000;China |
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| Abstract: | DNA fragments of about 220bp and 330bp were amplified by PCR using two pairs of specific primers that were designed from the ribosomal DNA (rNDA). The genomic DNA of Bursaphelenchus xylophilus and Bursaphelenchus mucronatus originated from Japan, United States and Portugal was used as PCR template. This technique can be used to identify the adults or larval of Bursaphelenchus xylophilus exactly. |
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| Keywords: | plant quarantine Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle rDNA-ITS1 PCR |
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