玉米骨干自交系幼胚再生体系的建立

以玉米骨干自交系‘87-1’、‘综3’、‘137’和‘K12’的幼胚为外植体,研究了影响玉米幼胚再生体系建立的相关因素.结果表明:87-1幼胚在附加500 mg.L-1L-Pro5、00 mg.L-1L-Asn和2.5 mg.L-12,4-D的N6B5基本培养基上愈伤组织诱导率最高,平均可高达98.1%;初级愈伤组织在附加500 mg.L-1L-Pro5、00 mg.L-1L-Asn和1.25 mg.L-12,4-D的N6B5继代培养基上胚性愈伤组织诱导率最高,平均可高达65.6%.在相同诱导、继代培养基和相同培养条件下,4个不同基因型玉米骨干自交系的愈伤组织诱导率无显著性差异,而其胚性愈伤组织诱导率具有显著性差异.继代培养次数对绿苗分化率也产生重要影响,具有显著性差异,其中,1次继代分化率最高;1/2MS培养基附加0.2 mg.L-1KT更有利于分化苗生根.

Establishment of plant regeneration system from immature embryos of maize (Zea mays L.) skeleton inbred lines

Immature embryos of maize skeleton inbred of 87-1, Zong3, 137 and K12 were used as explants to study the regeneration system of maize immature embryos, and the regenerative plants were obtained. The results indicated that the highest frequency of primary callus inducted from immature embryos of maize inbred line 87-1 was obtained on N6B5 medium supplemented with 500 mg·L~(-1) L-pro,500 mg·L~(-1) L-Asn and 2.5 mg·L~(-1) 2,4-D, which was reached to 98.1 %. The highest frequency of embryogenic callus formatted from primary callus were obtained on N6B5 medium supplemented with 500 mg·L~(-1) L-pro, 500 mg·L~(-1) L-Asn and 1.25 mg·L~(-1) 2,4-D, which was reached to 65.6 %. There was no significant differences among the primary callus induction frequency of 4 genotypes under the same culture condition, but the embryogenic callus formation frequencies were significantly different. The effects of subculture times on the frequency of plant regeneration were also significant different, and the frequency of plant regeneration was highest at only once subculture.1/2 MS medium supplemented with 0.2 mg·L~(-1) KT was benefit to the generation of root.