Up-regulation of HO-1 protects ADSCs under serum-free and hypoxic conditions |
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Authors: | ZHOU Zhong-xin CHEN Yi DAI Chuan-zhong WU Wei-heng |
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Institution: | 1.Department of Cardiothoracic Surgery, Affiliated Hospital of Xuzhou Medical College;2.Cardiovascular Institute, Xuzhou Medical College, Xuzhou 221002, China;3.Department of Cardiology, Second Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China. |
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Abstract: | AIM:To investigate the protective effects of endogenous heme oxygenase 1 (HO-1) induced by cobalt protoporphyrin (Copp, a HO-1 inducer) on adipose tissue-derived stromal cells (ADSCs) under the condition of serum-free and hypoxia. METHODS:The ADSCs were isolated from SD rat and cultured. The cell apoptotic rate was detected by DAPI staining. The protein expression of HO-1, NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC) and cleaved caspase-1 in ADSCs was messured by Western blotting. IL-1β level in supernatant was determined by ELISA. The level of intracellular reactive oxygen species (ROS) was detected using DCFH-DA. RESULTS:The up-regulation of HO -1 was induced by CoPP in a dose dependent manner and was most significant at 20 μmol/L. The increased expression of HO-1 induced by CoPP significantly reduced the apoptotic rate of ADSCs, intracellular ROS level and IL-1β secretion, and inhibited the overexpression of NLRP3, ASC and cleaved caspase-1 under serum and oxygen deprivation. These protective effects were reversed by zinc protoporphyrin (ZnPP, an HO-1 inhibitor) given simultaneously. CONCLUSION: The up-regulation of HO -1 expression induced by CoPP plays protective effect on ADSCs under the condition of serum and oxygen deprivation via inhibiting the activation of NLRP3 inflammasome and reducing IL-1β secretion. |
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Keywords: | Adipose tissue-derived stromal cells Heme oxygenase 1 Cobalt protoporphyrin Apoptosis |
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