Chloride currents activated by cisplatin in poorly differentiated naso-pharyngeal carcinoma cells are not Ca2+-activated chloride currents |
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Authors: | YANG Xiao-ya LIU Mei WU Jia-bao LAI Zhou-yi WANG Yuan FAN Ai-hui ZHU Lin-yan MAO Jian-wen WANG Li-wei CHEN Li-xin |
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Affiliation: | 1.Department of Physiology, College of Health Sciences, Guangzhou Medical University, Guangzhou 510180, China;2.Department of Pharmacology, 3Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China;4.Department of Physiology, Guangdong Medical College, Dongguan 523808, China;5.Department of Biology, Guangdong Pharmaceutical University, Guangzhou 510006, China. |
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Abstract: | AIM:To investigate the type of chloride channel activated by cisplatin in poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z cells). METHODS:The technique of whole-cell patch-clamp was used to investigate the role of Ca2+ in the activation of cisplatin-activated chloride currents and to analyze the effect of hypertonic stress on these currents in CNE-2Z cells. RESULTS:Chloride currents were induced when the cells were exposed to the calcium-free cisplatin solution, showing the similar density to the currents induced by cisplatin with the presence of extracellular calcium. However, the latency and the peak time of cisplatin-activated currents in the absence of extracellular calcium were prolonged. The activation of cisplatin-activated chloride currents was insensitive to the depletion of intra- and extracellular calcium. Calcium channel antagonist nifedipine had no effect on the cisplatin-activated chloride currents, while hypertonic solution completely inhibited those currents. CONCLUSION:The cisplatin-activated chloride currents are independent on intra/extracellular calcium. The chloride channels activated by cisplatin are not calcium-activated chloride channels, but are probably volume-sensitive chloride channels. |
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Keywords: | Cisplatin Nasopharyngeal neoplasms Chloride channels Calcium |
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