藏绵羊肺脏中链球菌的分离鉴定及耐药性分析

本试验通过无菌采集西藏地区绵羊肺脏，然后进行细菌分离、纯化及PCR鉴定；同时，对分离的链球菌菌株进行药物敏感性研究，以期为生产实践中对该病原的防制提供参考信息。结果显示，纯化所得到的细菌呈革兰氏阳性链球状，经PCR、克隆、测序鉴定为链球菌；药敏试验结果显示分离菌株对复方新诺明、恩诺沙星、庆大霉素及四环素等大部分药物敏感。本研究为西藏地区绵羊链球菌病的有效防制提供参考。

Isolation,Idetification and Drug Resistance Analysis of Streptococcus from Lungs of Tibetan Sheep

The lung samples with pneumoniae were collected from Tibetan sheep. Bacterial isolation and identification were performed by culture and PCR methods. Antibiotic sensitivity of the isolates was determined, so as to provide useful information for treatment of the disease. The results showed that the isolated bacteria were gram-positive and chain-forming coccus-shaped proven to be Streptococcus by specific PCR, clone and sequence. It was also demonstrated that the isolates were sensitivity to most of antibiotics, especially for trimethoprim-sulfamethoxazole, enrofloxacin,gentamicin and tetracycline. The results obtained in this study provided useful basis for control and treatment of Streptococcus infection of sheep in Tibetan.