X-ray irradiation increases sensitivity of myocardium to ischemia/reperfusion injury in rats |
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Authors: | WANG Jie LU Ke-yi ZHOU Jun-tao LI Xian-feng |
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Affiliation: | 1.Department of Neurology, 2.Department of Nuclear Medicine, 3.Department of Radiotherapy, First Hospital of Shanxi Medical University, Taiyuan 030001, China. |
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Abstract: | AIM:To observe the sensitivity of myocardium to ischemia/reperfusion (I/R) injury in the rats with chest radiotherapy. METHODS:The radiation-induced heart disease model was established by local 20 Gy of X-ray irradiation in the chest. Male Wistar rats (n=42) were randomly divided into 6 groups:sham trauma group, trauma group, sham trauma+sham operation group, sham trauma +I/R group, trauma+sham operation group and trauma+I/R group. The rats were subjected to 30 min of ischemia and 1 h of reperfusion 2 week after trauma. The left ventricular developed pressure (LVDP) and ±dp/dtmax were recorded by BL-410 biological signal recording and analysis system. The serum cardiac troponin I (cTnI) and creatine kinase isoenzyme (CK-MB) were measured by ELISA. The myocardial infarct size was determined by nitroblue tetrazolium(NBT) staining method and BI2000 image analysis software. RESULTS:Compared with sham trauma+I/R group, significant decreases in LVDP and ±dp/dtmax were observed in trauma+I/R group (P<0.01) with significant increases in the infarct size and the concentrations of cTnI and CK-MB (P<0.01). CONCLUSION:Chest X-ray irradiation increases the sensitivity of myocardium to I/R injury in rats. |
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Keywords: | Radiation-induced heart disease Myocardial ischemia Reperfusion injury Sensitivity |
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