| ASCL2基因在体细胞克隆牛和自然繁殖牛肺脏中的甲基化分析 |
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| 引用本文： | 王梦楠,张明月,李宁,李世杰. ASCL2基因在体细胞克隆牛和自然繁殖牛肺脏中的甲基化分析[J]. 中国畜牧兽医, 2014, 41(8): 71-75 |
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| 作者姓名： | 王梦楠 张明月 李宁 李世杰 |
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| 作者单位： | 1.河北农业大学生命科学学院，河北保定 071001；2.中国农业大学农业生物技术国家重点实验室，北京 100193 |
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| 基金项目： | 国家自然科学基金（31372312）；河北省自然基金项目（C2011204001）。 |
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| 摘 要： | 为探讨ASCL2基因在体细胞克隆牛中的重编程状态，本研究通过亚硫酸氢盐测序法（BSP）对体细胞克隆牛和自然繁殖牛肺脏中ASCL2基因启动子区CpG岛上的CpG位点进行了甲基化状态分析。结果显示，体细胞克隆牛的甲基化水平（28.41%）显著高于正常对照组（7.62%）（P＜0.05）。推测ASCL2基因的异常甲基化可能是造成体细胞克隆牛新生死亡及肺脏发育异常的原因之一。
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DNA Methylation Status of ASCL2 Gene in Lungs of SCNT Cattle and Naturally Reproduced Cattle |
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| WANG Meng-nan,ZHANG Ming-yue,LI Ning,LI Shi-jie. DNA Methylation Status of ASCL2 Gene in Lungs of SCNT Cattle and Naturally Reproduced Cattle[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(8): 71-75 |
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| Authors: | WANG Meng-nan ZHANG Ming-yue LI Ning LI Shi-jie |
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| Affiliation: | 1.College of Life Science，Hebei Agricultural University，Baoding 071001，China；2.State Key Laboratory for Agrobiotechnology，China Agricultural University，Beijing 100193，China |
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| Abstract: | In an effort to determine the reprogramming of ASCL2 gene in SCNT bovines，the bisulfite sequencing method （BSP） was used to analyze the DNA methylation status in promoter region of ASCL2 gene in lung tissues of SCNT cattle and natural breeding cattle.The results showed that the methylation level of ASCL2 promoter exhibited seriously hypermethylated in SCNT bovines （28.41%） compared with naturally breed bovines （7.62%） （P＜0.05）. Our results suggested that the abnormal DNA methylation of ASCL2 might be associated with the defects of lung organs in died SCNT bovines，which was one of the main reasons for neonatal death of SCNT animals. |
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