土槿皮乙酸通过P53和caspase蛋白抑制人乳腺癌细胞MCF-7生长机制研究

MCF-7细胞是caspase 3功能缺失的乳腺癌细胞，本研究结果发现土槿皮乙酸(PAB)可激活caspase 3 的上游蛋白caspase 8 和caspase 9来诱导细胞凋亡，同时PAB通过P53蛋白诱导人乳腺癌细胞MCF-7凋亡。本试验结果为进一步阐明PAB抗乳腺癌奠定了基础。

Study on Pseudolaric Acid B Inhibited Cell Growth through P53, Caspase 8 and Caspase 9 in MCF-7 Cells

The assay was aimed to study the inhibitory mechanism of pseudolaric acid B (PAB) in MCF-7 cell line. MCF-7 cell was human breast cancer cell lacking of caspase 3 function. In this study, it was found that PAB activated caspase 8 and caspase 9 to induce cell apoptosis. Meanwhile PAB up-regulated P53 protein expression. Therefore PAB induced MCF-7 cell apoptosis through increasing P53 protein expression and promoting the activation of caspase 8 and caspase 9.