副猪嗜血杆菌SC096株荚膜多糖及其抗血清的制备

为获得副猪嗜血杆菌荚膜多糖及其抗血清,本试验采用热酚法提取副猪嗜血杆菌SC096株荚膜多糖,并进行SDS-PAGE及银染检测,然后用荚膜多糖制备的油乳疫苗免疫家兔制备抗血清,并进行琼脂扩散试验及间接血凝试验检测.试验结果显示,热酚法可制备高浓度的荚膜多糖;SDS-PAGE电泳及银染结果显示条带呈弥散性分布,荚膜多糖分子质量55 ku左右;琼脂扩散试验结果呈阳性;间接血凝试验中红细胞发生高达10孔滴度的凝集现象.副猪嗜血杆菌SC096株荚膜多糖的提取及其抗血清的制备,为其他含荚膜的细菌抗血清的制备提供了方法和理论依据, 也为细菌荚膜多糖抗原性及其疫苗研究奠定了基础.

Extraction of Capsular Polysaccharide from Haemophilus parasuis SC096 Strain and Preparation of its Antiserum

With the hot phenol method to extract the capsular polysaccharide (CPS), CPS was detected by SDS-PAGE and silver stain, and subsequently made emulsion with Freund's adjuvant.The antiserum was prepared by immunizing rabbits, and examined by agar diffusion test and indirect hemagglutination assay (IHA).The results showed that a high concentration of CPS was obtained by hot phenol method, SDS-PAGE electrophoresis showed that bands were diffuse distribution and the molecular mass was about 55 ku. Agar diffusion test result was positive and red blood cells occurred up to 10 hole drop degrees by IHA.The extraction of CPS and preparation of antiserum of Haemophilus parasuis SC096 strain provided a preparation method and the theoretical basis for a variety of other bacteria containing capsule, and made the foundation for study of antigenicity and vaccine of bacterial CPS.