α7nAChR is involved in anti-inflammation of physiological concentrations of glucocorticoids |
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Authors: | CHEN Yi-yu L Jun-hua ZHANG Mei |
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Institution: | 1.Department of Pharmacology, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 510082, China;2.Department of Pharmacology, Jinan University College of Pharmacy, Guangzhou 510630, China. |
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Abstract: | AIM: To explore the role of α7 nicotinic acetylcholine receptor (α7nAChR) in anti-inflammation of glucocorticoids (GCs) at physiological concentrations. METHODS: MTT assay was used to measure the viability of BV-2 cells, which were processed by hydrocortisone at different concentrations. On the basis of inflammatory model induced by LPS in BV-2 cells, experimental groups were divided as follows: (1) control; (2) LPS; (3) GCs+LPS; (4) methyllycaconitine (MLA)+GCs+LPS. The levels of TNF-α and IL-1β in the cell supernatants were detected by ELISA. RESULTS: Hydrocortisone at concentrations of 2 000 and 1 000 nmol/L decreased the cell viability to (76.9±5.5)% and (90.8±7.3)%, respectively, indicating the cellular injury by GCs at over-physiological doses. LPS significantly induced the releases of TNF-α and IL-1β in a time- and dose-dependent manner in BV-2 cells. Hydrocortisone at physiological concentrations (500 and 250 nmol/L) reduced the releases of TNF-α and IL-1β in BV-2 cells stimulated by LPS, and MLA at concentration of 10 nmol/L antagonized the anti-inflammatory effect of GCs. CONCLUSION: α7nAChR is involved in the anti-inflammatory effect of the physiological concentrations of GCs. |
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Keywords: | Glucocorticoids α7 nicotinic acetylcholine receptor Microglia Lipopolysaccharides |
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