甘蓝无蜡粉亮绿突变体材料LD10遗传规律及分子标记研究

为了明确结球甘蓝无蜡粉亮绿突变体材料LD10 的特征特性及遗传规律，测定了LD10 的主要农艺性状和营养物质
含量；对LD10 和普通材料21-3 的叶面超微结构进行了观察比较；以LD10 和21-3 为亲本构建6 世代群体，对LD10 无蜡
粉亮绿性状遗传规律进行分析。结果表明：无蜡粉亮绿突变体材料LD10 叶面蜡粉呈小颗粒状结构，缺失严重，而普通材料
21-3 叶面蜡粉呈长棒状结构，含量丰富，二者具有明显差异；LD10 的无蜡粉亮绿性状由1 个隐性基因（cgl-4）控制，其与
SSR 标记LT-SSR16 紧密连锁，遗传距离为4.7 cM。用LD10 与另一单基因隐性遗传的无蜡粉亮绿突变体材料10Q-961 杂交
获得F1 群体，F1 群体单株全部表现为叶面有蜡粉，推断LD10 与10Q-961 的无蜡粉亮绿基因位于不同的遗传位点。

Studies on Inheritance and Molecular Marker in Cabbage Glossy Wax-less Mutant LD10

To clarify the characteristics and inheritance of cabbage（Brassica oleracea L. var. capitata L.）
glossy wax-less mutant LD10，the major agronomic characteristics and nutrient contents of LD10 were tested，
and ultra-structures of leaves in mutant material LD10 and normal material 21-3 were measured，observed
and comparatively analyzed. LD10 and 21-3 were taken as parent to construct 6 generations population，and
inheritance analysis onthe glossy wax-less characteristics of LD10 was conducted. The results showed that there
was little wax on the leaves of LD10，while the wax on the leaves of 21-3 was abundent，and the granular wax in
LD10 was of long stick shape，significantly different from the wax in 21-3. The glossy wax-less trait in cabbage
LD10 was controlled by one single recessive gene named cgl-4. One molecular marker（LT-SSR16）linked to
cgl-4 with genetic distances 4.7 cM. Another F₁ population obtained through crossing LD10 and 10Q-961 showed
that the genes leading to glossy wax-less trait were at different locations in the two glossy wax-less materials.