Effects of SUMOylation on IKKγ/NF-κB signaling in cultured rat glomerular mesangial cells treated with high glucose

AIM：To investigate the effects of SUMOylation on IκB kinase γ (IKKγ)/NF-κB signaling in cultured rat glomerular mesangial cells (GMCs) treated with high glucose. METHODS：Cultured HBZY-1 rat GMCs were divided into normal glucose group and high glucose groups, and mannitol was used for osmotic control. The expression of SUMO1, SUMO2/3, IKKγ and NF-κB p65 was measured by Western blotting and RT-PCR. Interaction between SUMO and IKKγ was detected by co-immunoprecipitation. RESULTS：Compared with normal glucose group, the expression of SUMO and NF-κB p65 was increased in high glucose groups in a dose- and time-dependent manner. The expression of IKKγ was not changed by high glucose. The SUMOylation of IKKγ in high glucose groups was significantly decreased as compared with normal glucose group. CONCLUSION:High glucose obviously changes the interaction between SUMO and IKKγ in cultured rat mesangial cells, which may be involved in the activation of NF-κB by taking a special influence on the SUMOylation of IKKγ/NF-κB signaling molecules.