犬弓形虫抗体间接ELISA检测方法的建立

试验以利用重组弓形虫SAG1基因转染蜥蜴利什曼原虫所表达获得的目的蛋白作为包被抗原，建立了一种快速、特异、可检测犬弓形虫抗体的间接ELISA方法。确定了抗原最佳包被浓度为6.75 μg/mL，血清最佳稀释度为1∶100，对已知阳性血清检测的下限可达1∶6400，批间和批内重复性试验的变异系数均小于10%，包被抗原的酶标板在4、-20 ℃环境中可保存8个月以上。建立的间接ELISA方法应用于犬弓形虫抗体的检测具有较好的敏感性及特异性。

Establishment of Indirect ELISA Detection Method for Toxoplasma gondii Antibody in Dogs

The assay was aimed to establish a rapid and specific indirect ELISA method for canine Toxoplasma gondii antibody detection，using expressed protein of recombinant Toxoplasma gondii SAG1 gene transfected into Leishmania tarentolae as the coating antigen. The optimal antigen coating concentration was 6.75 μg/mL, serum optimal dilution was 1∶100, the known positive serum detection was more than 1∶6400, the coefficients of variation for intra-assay and inter-assay were both less than 10%, and the coated ELISA plate could be stored more than 8 months at 4 and -20 ℃. The present indirect ELISA detection method was sensitivity and specificity for detecting Toxoplasma gondii antibody in canine.