Expression and function of calcium-sensing receptor in mouse embryonic stem cells |
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| Authors: | SUN Jian YU Jin-feng LUAN Hai-rong LI Shuang XU Xiao-yi HE Zhi-peng WEI Tao LI Li XU Chang-qing |
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| Institution: | 1.Mudanjiang Medical College, Mudanjiang 157011, China;2.Department of Pathophysiology, Harbin Medical University, Harbin 150081, China. |
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| Abstract: | AIM: To observe the functional expression of calcium-sensing receptor (CaSR) in the mouse embryonic stem cells (mESCs). METHODS: The expression and distribution of CaSR were detected by Western blotting and immunofluorescence observation in 129 mouse ES-D3 cells. The intracellular concentration of free calcium (［Ca2+］i) was determined by confocal laser scanning microscopy. The cell viability was analyzed by MTT assay and flow cytometry. RESULTS: CaSR protein was expressed in mESCs. Extracellular calcium or neomycin significantly increased the expression of CaSR and ［Ca2+］i. Neomycin increased the cell viability, up-regulated the protein expression of p-ERK2. These effects of neomycin were inhibited by NPS2390. CONCLUSION: CaSR is expressed in mESCs. The activation of CaSR is involved in the proliferation of mESCs. |
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| Keywords: | Calcium-sensing receptor Mouse embryonic stem cells Cell proliferation |
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