芥菜种质资源遗传多样性和亲缘关系的SRAP 分析

利用SRAP 标记对111 份芥菜种质的遗传多样性进行了分析，并记载了其形态特征。从300 对SRAP 引物中筛选出21
对多态性明显、条带清晰、反应稳定的引物组合，共扩增出150 条条带，其中106 条为多态性条带，多态性比率为70.67%。
SRAP 聚类分析结果表明，111 份芥菜材料的遗传相似系数在0.38～0.89 之间，分为5 大类和若干亚类。对芥菜的叶片性状及根、
茎、叶、薹的发育状况等农艺性状进行调查分析，结果表明111 份芥菜材料的遗传相似系数在0.39～1.00 之间，分为4 大类和
若干亚类。这两种方法的聚类结果基本一致，表明SRAP 标记可以应用于芥菜种质资源遗传多样性和亲缘关系研究。

SRAP Analysis of Mustard Germplasm Genetic Diversity and GeneticRelationship

Abstract：The paper analyzed the genetic diversity of 111 mustards〔 Brassica juncea（L.）Czern. et Coss.〕
materials using SRAP markers，and recorded their morphological characteristics．21 primer combinations with high
polymorphism were screened from 300 SRAP primer combinations．106 polymorphic bands among 150 bands were
amplified by 21 SRAP primer combinations，and the polymorphic rate was 70.67%．The SRAP clustering results
showed that the genetic similarity of 111 mustards ranged from 0.38 to 0.89，and 111 mustards were divided into
5 groups and several subgroups．The agronomic traits including leaf blade traits，and root，stem，leaf，bolting
development were investigated and analyzed．The results showed that the genetic similarity of 111 mustards ranged
from 0.39 to 1.00，and they could be divided into 4 groups and several subgroups．These two results were nearly the
same，indicating that SRAP markers could be applied in studying the genetic diversity and relationships of mustard
germplasm resources．