| 猪毛色基因黑色素受体1的基因型分析 |
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| 引用本文： | 印崇,高峰,李傲楠,张成才,周梦娇,白春艳,孙博兴,赵志辉,邢沈阳. 猪毛色基因黑色素受体1的基因型分析[J]. 中国畜牧兽医, 2014, 41(11): 44-49 |
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| 作者姓名： | 印崇 高峰 李傲楠 张成才 周梦娇 白春艳 孙博兴 赵志辉 邢沈阳 |
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| 作者单位： | 1. 吉林大学农学部动物科学学院, 吉林长春 130062;2. 西北工业大学生命学院, 陕西西安 710072 |
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| 基金项目： | 吉林省科技厅农业处资助项目(20120224);长春市科技局农业处资助项目(2012231)。 |
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| 摘 要： | 为检测黑色素受体1(melanocortin receptor 1,MC1R)基因型在不同毛色猪种中的分布,研究该基因在猪毛色决定中的地位和作用,本试验使用PCR-SSCP和PCR-RFLP方法对军牧1号白猪、杜洛克猪、西藏小型猪和大白猪的MC1R基因型进行了检测。结果显示,长白猪、大白猪存在nt894insCC和G1197A突变;杜洛克猪存在G668C、C1318T和G1554A突变;西藏小型猪存在C1318T和G1554A突变,而在nt894insCC位点则表现出其他基因型。通过对4个猪种的MC1R基因型的检测,为研究MC1R基因对毛色的作用机理奠定了基础。
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| 关 键 词： | MC1R基因 毛色 猪 |
The Genotype Analysis of Coat Color Gene MC1R in Swine |
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| YIN Chong,GAO Feng,LI Ao-nan,ZHANG Cheng-cai,ZHOU Meng-jiao,BAI Chun-yan,SUN Bo-xing,ZHAO Zhi-hui,XING Shen-yang. The Genotype Analysis of Coat Color Gene MC1R in Swine[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(11): 44-49 |
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| Authors: | YIN Chong GAO Feng LI Ao-nan ZHANG Cheng-cai ZHOU Meng-jiao BAI Chun-yan SUN Bo-xing ZHAO Zhi-hui XING Shen-yang |
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| Affiliation: | 1. College of Animal Science, Jilin University, Changchun 130062, China;2. School of Life Science, Northwestern Polytechnical University, Xi'an 710072, China |
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| Abstract: | For analysis melanocortin receptor 1(MC1R) genotype in swine spices with different coat color and study the gene functions on swine coat color. This research analysed the genotype of MC1R in Junmu 1 White pigs, Durocs pigs, Tibet mini-pigs and Large White pigs with PCR-SSCP and PCR-RFLP technique. The result showed that Junmu 1 White pigs and Large White pigs presented mutation on nt894insCC and G1197A site, Durocs pigs presented mutation on G668C, C1318T, and G1554A site, Tibet mini-pigs presented mutation on C1318T and G1554A site, with a different genotype on nt894insCC site. The MC1R genotype result provided a basis for studying MC1R regulation mechanism to swine coat color. |
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| Keywords: | MC1R gene coat color swine |
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