| 饲料中沙丁胺醇酶联免疫前处理方法的探究 |
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| 引用本文： | 栗静雅,王战辉,王文珺,江海洋,沈建忠.饲料中沙丁胺醇酶联免疫前处理方法的探究[J].中国畜牧兽医,2014,41(11):179-183. |
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| 作者姓名： | 栗静雅 王战辉 王文珺 江海洋 沈建忠 |
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| 作者单位： | 1. 中国农业大学动物医学院, 北京 100193;2. 国家兽药安全评价中心, 北京 100193;3. 北京维德维康生物技术有限公司, 北京 100085 |
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| 基金项目： | 公益性行业(农业)科研专项(2012104003-5)。 |
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| 摘 要： | 为探讨酶联免疫法(ELISA)检测饲料中沙丁胺醇(salbutamol,SAL)的前处理方法,试验对提取液的pH、离子强度、有机溶剂浓度及样品稀释倍数等条件进行了考察,选取最适宜的提取液条件对2种不同类型的饲料样本进行提取,用ELISA测定样品中沙丁胺醇的残留量。结果表明,沙丁胺醇标准曲线的IC50值为0.606 ng/mL,线性范围为0.221～1.658 ng/mL,R2=0.9998,提取液的pH为7.5,PBS缓冲液最优浓度为0.06 mol/L,稀释倍数为10倍,2种不同类型的饲料样本的检测限(LOD)为5.0 ng/mL。当沙丁胺醇的添加浓度为5、10 μg/kg时,该方法的添加回收率为77%～110%,变异系数<8%。
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| 关 键 词： | 酶联免疫法 沙丁胺醇 前处理 饲料 |
| 收稿时间： | 2014-05-07 |
Study on Preparation Treatment for Salbutamol in Feed by Enzyme-linked Immunosorbent Assay |
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| LI Jing-ya,WANG Zhan-hui,WANG Wen-jun,JIANG Hai-yang,SHEN Jian-zhong.Study on Preparation Treatment for Salbutamol in Feed by Enzyme-linked Immunosorbent Assay[J].China Animal Husbandry & Veterinary Medicine,2014,41(11):179-183. |
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| Authors: | LI Jing-ya WANG Zhan-hui WANG Wen-jun JIANG Hai-yang SHEN Jian-zhong |
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| Institution: | 1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;2. National Reference Laboratory for Residues of Veterinary Drugs, Beijing 100193, China;3. Beijing WDWK Biotechnology Co., Ltd., Beijing 100085, China |
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| Abstract: | Objective of the study was to develop a preparation treatment for salbutamol in feed by enzyme-linked immunosorbent assay (ELISA).The assay optimized the influence factor of the preparation and used to extract salbutamol residues in feed.The results showed that the inhibition concentration (IC50) for the developed ELISA was 0.606 ng/mL with the detection range of 0.221 to 1.658 ng/mL and R2 was 0.9998.After optimization,pH 7.5,0.06 mol/L PBS with the dilution ratio of 10 was selected to extract salbutamol residues in feed and the limit of detection (LOD) was 5.0 ng/mL in feed.The recoveries ranged from 77% to 110% for the spiked samples (5 and 10 μg/kg),and RSD was less than 8%. |
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| Keywords: | enzyme-linked immunosorbent assay (ELISA) salbutamol preparation treatment feed |
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