| 贵州地方鸡种的遗传变异研究 |
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| 引用本文： | 王文涛,林家栋,陈眷华,张福平.贵州地方鸡种的遗传变异研究[J].中国畜牧兽医,2014,41(4):185-188. |
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| 作者姓名： | 王文涛 林家栋 陈眷华 张福平 |
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| 作者单位： | （1.贵州省清镇市农业局, 贵州清镇 551400;2.贵州大学动物科学学院, 贵州贵阳 550025) |
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| 基金项目： | 贵州省科技攻关项目(黔科合NZ字［2012］3007号)。 |
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| 摘 要： | 为了研究贵州地方鸡种的遗传多样性与系统进化，试验通过PCR直接测序的方法，测定了6个贵州地方鸡种的90个个体线粒体DNA控制区部分序列，并进行了序列分析。结果表明，DNA控制区序列长度为780 bp，A、G、C、T 4种核苷酸的平均比例分别为26.14%、13.38%、25.98%、34.50%；共发现40个变异位点(不包含种内变异位点)，占分析位点总数的5.13%；共确定30个单倍型，6个群体内单倍型变异度总体为0.914，变化范围为0.506～0.976。6个群体间表现出显著的遗传分化和较高水平的群体遗传多态性。
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| 收稿时间： | 2013-10-18 |
Study on Genetic Diversity of Domestic Chicken in Guizhou |
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| WANG Wen-tao,LIN Jia-dong,CHEN Juan-hua,ZHANG Fu-ping.Study on Genetic Diversity of Domestic Chicken in Guizhou[J].China Animal Husbandry & Veterinary Medicine,2014,41(4):185-188. |
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| Authors: | WANG Wen-tao LIN Jia-dong CHEN Juan-hua ZHANG Fu-ping |
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| Institution: | (1. Qingzhen Agricultural Bureau, Guizhou Province, Qingzhen 551400, China;2. College of Animal Science, Guizhou University, Guiyang 550025,China) |
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| Abstract: | PCR and direct sequencing methods were used to analyze the genetic diversity and systematic evolution in Guizhou domestic fowls by determining a 780 bp DNA fragment of control region of mtDNA from 90 individuals of 6 Guizhou domestic chicken breeds. The percentage of nucleotide A, G, C and T were 26.14%，13.38%，25.98% and 34.50%, respectively. There were 40 polymorphic sites accounting for 5.13% of total analyzed sites. The diversity of haplotypes in 6 Guizhou domestic chicken breeds was ranged from 0.506 to 0.976, which showed significant genetic differentiation and higher levels of population genetic polymorphism. |
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