| 小尾寒羊白介素1β全长cDNA序列克隆及生物信息学分析 |
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| 引用本文： | 郭兴,刘楠楠,陈晓峰,张文惠,胡盼,李岩松,李兆辉,王光明,卢士英,周玉,柳增善,任洪林. 小尾寒羊白介素1β全长cDNA序列克隆及生物信息学分析[J]. 中国畜牧兽医, 2014, 41(12): 20-24 |
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| 作者姓名： | 郭兴 刘楠楠 陈晓峰 张文惠 胡盼 李岩松 李兆辉 王光明 卢士英 周玉 柳增善 任洪林 |
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| 作者单位： | 1. 人兽共患病研究教育部重点实验室, 吉林大学人兽共患病研究所, 吉林大学动物医学学院, 吉林长春 130062;2. 福建省亚热带植物研究所, 福建厦门 361006 |
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| 基金项目： | 国家自然科学基金(30901070). |
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| 摘 要： | 本研究旨在克隆白介素1β(IL-1β)全长cDNA,为深入研究其生物学功能提供全长cDNA信息和试验材料.本试验利用前人构建的布鲁氏菌强、弱毒株感染小尾寒羊白细胞层抑制性差减杂交(SSH)cDNA文库,获得小尾寒羊IL-1β部分cDNA序列信息,利用cDNA末端快速扩增技术(RACE),克隆小尾寒羊IL-1β全长cDNA,并申请GenBank登录号KC425612.2.该cDNA全长1494 bp,含有801 bp开放阅读框(ORF),可编码266个氨基酸残基,预测其编码蛋白分子质量为30.622 ku,与林麝、牛、海豚、虎鲸的IL-1β一致性较高,亲缘关系较近.
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| 关 键 词： | 白介素1β 小尾寒羊 克隆 生物信息学 分析 |
| 收稿时间： | 2014-07-21 |
Cloning and Bioinformatics Analysis of Interleukin 1 Beta Full-length cDNA of Small-tailed Han Sheep |
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| GUO Xing,LIU Nan-nan,CHEN Xiao-feng,ZHANG Wen-hui,HU Pan,LI Yan-song,LI Zhao-hui,WANG Guang-ming,LU Shi-ying,ZHOU Yu,LIU Zeng-shan,REN Hong-lin. Cloning and Bioinformatics Analysis of Interleukin 1 Beta Full-length cDNA of Small-tailed Han Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(12): 20-24 |
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| Authors: | GUO Xing LIU Nan-nan CHEN Xiao-feng ZHANG Wen-hui HU Pan LI Yan-song LI Zhao-hui WANG Guang-ming LU Shi-ying ZHOU Yu LIU Zeng-shan REN Hong-lin |
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| Affiliation: | 1. Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China;2. Fujian Institute of Subtropical Botany, Xiamen 361006, China |
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| Abstract: | This study was aimed at cloning the full length cDNA of interleukin 1 beta (IL-1β) and providing full length cDNA information and experimental materials for further research on its biology function. The full-length cDNA of IL-1β from Small-tailed Han sheep was cloned according to the SSH cDNA library, using 5'-RACE technique. The full cDNA sequence was obtained and indexed in GenBank database with the number of KC425612.2. The results showed that the full-length IL-1β cDNA consisted of 1494 bp nucleic acids with the ORF of 801 bp encoding 266 amino acid residues of the predicted protein molecular weight of 30.622 ku, and the predicted protein IL-1β of Small-tailed Han sheep showed high homology with those of Moschus berezovskii, Bos taurus, Tursiops truncate and Orcinus orca. |
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| Keywords: | IL-1β Small-tailed Han sheep clone bioinformatics analysis |
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