京海黄鸡甲状腺激素应答蛋白Spot14α基因外显子1多态性与屠体和腹脂性状的相关性分析

本研究旨在寻找甲状腺激素应答蛋白Spot14α(thyroid hormone responsive Spot14α，THRSPα)基因的多态位点，并分析其对京海黄鸡屠体和腹脂性状产生的影响。针对THRSPα基因外显子区,利用PCR-SSCP结合测序技术对379只140日龄京海黄鸡进行SNP检测，结果发现，该基因第1外显子区存在9 bp插入缺失多态位点，形成3种基因型：AA、AB和BB，2种等位基因：A、B，基因频率分别为0.4485、0.5515。相关分析结果表明，该位点对京海黄鸡140日龄活重、屠体性状（包括屠体重、头重、脚重、翅重、胸肌重、腿肌重、全净膛重、半净膛重）和腹脂性状均没有显著影响(P>0.05)。因此推断该多态位点在京海黄鸡分子辅助标记育种中不能作为屠体和腹脂性状筛选的候选分子辅助标记。

Relationship between Polymorphisms of Exon 1 of THRSPα Gene and the Carcass and Abdominal Fat Traits in Jinghai Yellow Chicken

The objective of this study was to find out the SNPs of THRSPα gene and analysed their effects on the carcass and abdominal fat traits in Jinghai Yellow chicken. This experiment aimed at the exon region of THRSPα gene, using PCR-SSCP combined with sequencing technology to detect the SNPs in 379 Jinghai Yellow chickens at 140 days old. We foud that there was a 9 bp del/ins mutation in the exon 1 area of the gene. For the THRSPα gene, three genotypes(AA, AB and BB) were detected in Jinghai Yellow chicken. The frequencies of A and B alleles were 0.4485 and 0.5515, respectively. The results of the correlation analysis showed that the polymorphsim site had no significant influence(P>0.05) on the 140 day weight, carcass(including carcass weight, head weight, feet weight, wing weight, chest muscle weight, leg muscle weight, eviscerated weight, half eviscerated weight) and abdominal fat traits. Therefore,we concluded that the polymorphsim site could’t be used as a potential marker of carcass and abdominal fat traits in Jinghai Yellow chicken molecular marker-assisted breeding.