补肾活血方对骨性关节炎模型SD大鼠基质金属蛋白酶-1及前列腺素E2的影响

本试验旨在观察补肾活血方对骨性关节炎模型SD大鼠关节软骨中基质金属蛋白酶-1（MMP-1）和关节液中前列腺素E₂（PGE₂）水平的影响，以探讨补肾活血方对骨性关节炎关节软骨的保护机制。将24只SPF级健康雌性SD大鼠随机分为假手术组、西药组和中药组，通过切除两侧卵巢并切断右侧膝交叉及内侧副韧带建立骨性关节炎动物模型。术后第4周开始，中药组灌服补肾活血中药，西药组灌服等量的西乐葆，假手术组灌服等量的生理盐水，于术后第8周处死动物，采用骨性关节炎软骨病理变化评价系统（OARSI）评价关节软骨的病变，采用光镜观察软骨细胞生长情况，运用免疫组化法测定关节软骨中MMP-1的含量，抽取关节液做PGE₂测定，对比各组数据。结果显示，假手术组、西药组、中药组软骨退变程度依次减轻，光镜下,假手术组、西药组仅见少量软骨细胞，而中药组见大量软骨细胞增生；西药组中各层软骨细胞几乎都可见大量的MMP-1阳性表达，中药组和假手术组MMP-1阳性表达极显著低于西药组（P＜0.01）；中药组和假手术组关节液中的PGE₂分别显著和极显著低于西药组（P＜0.05，P＜0.01）；中药组和西药组分别较其术后4周的结果极显著升高（P＜0.01）。补肾活血方能显著抑制骨性关节炎关节软骨中MMP-1的表达及降低关节液中的PGE₂水平，促进软骨细胞生长，以减缓骨性关节炎的发展，故具有治疗骨性关节炎的潜力。

Effect of Kidney-tonifying and Blood Circulation-promoting Recipes on Matrix Metalloproteinase-1 and Prostaglandin E2 in Osteoarthritis SD Rats

The study was aimed to observe the influence of Kidney-tonifying and Blood Circulation-promoting Recipes on the level of prostaglandin E₂(PGE₂) in joint fluid and matrix metalloproteinase-1(MMP-1) in articular cartilage of osteoarthritis model SD rats, so as to investigate the protection mechanism of articular cartilage of osteoarthritis by Kidney-tonifying and Blood Circulation-promoting Recipes. Twenty-four SD rats were randomly divided into three groups， sham operation group, traditional Chinese medicine(TCM) group and western medicine group, the sham operation group was treated with normal saline, the western medicine group was treated with celebrex, the Chinese medicine group was treated with Kidney-tonifying and Blood Circulation-promoting Recipes in 4 weeks after operation. In 8 weeks after operation, rats were sacrificed, with the OARSI to evaluate the lesions of articular cartilage, observed the growth of chondrocytes by light microscopy, using immunohistochemical method for determining the content of MMP-1 in articular cartilage in 4 weeks after operation, measuring PGE₂ in the fluid in 4 and 8 weeks after operation, compared the datas of the three groups. The cartilage degeneration of sham operation group, western medicine group and TCM group reduced sequentially, observed under light microscope, there were only a small amount of cartilage cells in the sham operation group and western medicine group, and a lot of cartilage cells were seen in the TCM group. In the western medicine group, almost all layers of chondrocytes could see a lot of positive expression of MMP-1, expression of MMP-1 in TCM group and sham operation group were extremely significantly lower than that of western medicine group(P＜0.01); PGE₂ of synovial fluid in TCM group and sham operation group were significantly and extremely significantly lower than that of western medicine group(P＜0.05, P＜0.01), respectively; compared with the ones which in the 4 weeks after operation the PGE₂ of synovial fluid in TCM group and western medicine group were extremely significantly increased(P＜0.01). Kidney-tonifying and Blood Circulation-promoting Recipes could significantly inhibit the expression of MMP-1 in osteoarthritis articular cartilage and reduce the level of PGE₂ in the joint fluid, promote the growth of chondrocytes, and to slow the progression of osteoarthritis, which had the potential for the treatment of osteoarthritis.