陆地棉GhbHLH4基因的克隆与功能分析

bHLH是一类存在于真核生物中的转录因子家族，其成员广泛参与到植物生长发育及抗逆过程之中。但是，在棉花中有关bHLH基因功能的研究报道不多。以陆地棉为材料分离克隆了GhbHLH4基因并对其功能进行了初步分析。结果表明：GhbHLH4基因启动子中含有大量与抗逆相关的顺式作用元件；转GhbHLH4基因的拟南芥植株与野生型相比叶片宽大、根系发达，转GhbHLH4基因植株能够耐受150 mmol/L NaCl并完成整个生长过程。研究结果为深入了解GhbHLH4基因耐盐的分子机理奠定了基础。

Cloning and Functional Analysis of GhbHLH4 Gene from Upland Cotton

bHLH is a superfamily in plant kingdom, whose members participate in the processes of plant growth, development, and stresses resistance. But up to now, the functions of bHLHs in cotton are less studied and reported. This paper took Gossypium hirsutum as material, seperates and clones gene|GhbHLH4, and carried out initial analysis on its function. The results showed that there are abundant stress|response relevant cis|elements in the promoter region of gene GhbHLH4. The leaves and roots of transgenic GhbHLH4 Arabidopsis plants were wider and longer than WT. Transgenic GhbHLH4 plants grew normally under long|time treatment of 150 mmol/L NaCl. The results of this study has laid a foundation for thorough understanding the molecular mechanism of salt tolerance in GhbHLH4 gene.