| 三穗鸭肌肉生长抑制素基因的生物信息学分析 |
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| 引用本文： | 易恒洁,李辉,杨胜林,赵忠海,彭邦星. 三穗鸭肌肉生长抑制素基因的生物信息学分析[J]. 中国畜牧兽医, 2014, 41(4): 1-6 |
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| 作者姓名： | 易恒洁 李辉 杨胜林 赵忠海 彭邦星 |
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| 作者单位： | （1.贵州大学动物科学学院，贵州贵阳 550025；2.贵州省动物遗传育种与繁殖重点实验室，贵州贵阳 550025；3.贵州大学高原山地动物遗传育种与繁殖教育部重点实验室，贵州贵阳 550025） |
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| 基金项目： | 教育部科学技术研究重点项目“贵州省地方鸭品种屠宰性状相关基因的克隆与SNPs检测”（211168）；贵州省科技厅农业重大专项［黔科合重大专项字（2012）6004号］；《三穗鸭国家标准》制定与养殖技术规程编制横向［H120183］。 |
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| 摘 要： | 采用PCR产物直接测序的方法获得肌肉生长抑制素（myostatin，MSTN）基因的编码区序列，并运用生物信息学方法对该基因编码蛋白的理化性质、信号肽、跨膜结构、二级结构、三级结构和功能结构域进行分析。结果表明，三穗鸭MSTN蛋白是一个疏水性不稳定蛋白，作为信号肽的可能性很大；含有22个α螺旋、26个β折叠、26个T转角、20个无规则卷曲，有1个跨膜结构，具有TGF-β的主要结构。研究结果为MSTN基因的进一步研究提供参考。
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| 关 键 词： | 三穗鸭 肌肉生长抑制素基因 生物信息学 |
| 收稿时间： | 2013-10-18 |
Bioinformatics Analysis of MSTN Gene in Sansui Duck |
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| YI Heng-jie,LI Hui,YANG Sheng-lin,ZHAO Zhong-hai,PENG Bang-xing. Bioinformatics Analysis of MSTN Gene in Sansui Duck[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(4): 1-6 |
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| Authors: | YI Heng-jie LI Hui YANG Sheng-lin ZHAO Zhong-hai PENG Bang-xing |
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| Affiliation: | (1.College of Animal Science, Guizhou University, Guiyang 550025,China；2.Key Laboratory of Animal Genetics, Breeding and Reproduction,Guizhou Province, Guiyang 550025,China；3.Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025,China) |
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| Abstract: | In this study, the coding region of MSTN gene was amplified by PCR and sequenced by direct sequencing. Some characters of the MSTN gene encoding protein, including the physico-chemical properties, singal peptide, ransmembrane structure, secondary structure, high structure and functional domains, were analyzed by bioinformatics tools. The results showed that MSTN protein of Sansui duck was a kind of labile protein with high hydrophobicity, which was probably being singnal peptide. The protein contained 22 α-helics,26 β-sheets,26 T-turns,20 irregular coils and 1 transmembrane domain, and it had the main feature of TGF-β superfamily. The result provided an important reference for its further research. |
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