Anti-inflammatory effects of PYNOD on lipopolysaccharide-stimulated BV2 microglial cells

AIM: To validate the anti-inflammatory effect of PYNOD on lipopolysaccharide (LPS)-stimulated BV2 microglial cells.METHODS: A specific GFP and PYNOD fluorescence expression vector pEGFP-C2-PYNOD driven by the promoter of CMV gene was constructed. The anti-inflammatory properties of PYNOD were studied using LPS-stimulated BV2 microglia model. The productions of nitric oxide (NO), inducible NO synthase (iNOS), interleukin-1β (IL-1β) which caspase-1 were evaluated as inflammatory parameters. RESULTS: Pretreatment with pEGFP-C2-PYNOD to BV2 microglia cells stimulated by LPS significantly inhibited the excessive productions of NO and IL-1β, which was associated with down-regulation of iNOS and caspase-1 at mRNA and protein levels. CONCLUSION: PYNOD might be useful for treating the inflammatory and deleterious effects of BV2 microglial cell activation in response to LPS stimulation.