猪呼吸道疾病综合征中性粒细胞cAMP-PDE和血浆cAMP、IL-6、TNF-α的变化及中药疗效研究

本试验通过观察呼吸道疾病综合征（PRDC）病猪中性粒细胞（PMN）环磷酸腺苷磷酸二酯酶（cAMP-PDE）活性和血浆cAMP、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）含量的变化及自拟中药汤剂对其的影响与疗效，以期为呼吸道炎症的病理机制与中药防制积累资料。将PRDC病猪随机分组，治疗组灌服由芦根、黄芪、白芍、甘草组成的1 g生药/mL中药，1.5 mL/kg，每天2次，连续给药10 d。停药后取全血检测各组外周血白细胞数量；并分离PMN，HPLC法检测cAMP-PDE活性；ELISA试剂盒测定血浆中cAMP、IL-6、TNF-α的含量。与健康对照组相比，患病组白细胞数量显著升高（P<0.05），cAMP-PDE活性和血浆TNF-α、IL-6含量均极显著升高（P<0.01）；治疗组TNF-α含量显著升高（P<0.05）。与患病组相比，治疗组的白细胞数量、cAMP-PDE活性和血浆TNF-α含量均显著降低（P<0.05），IL-6极显著降低（P<0.01）。结果表明PRDC病猪存在外周血白细胞数量、PMN cAMP-PDE活性和血浆IL-6、TNF-α含量升高的异常变化，自拟中药汤剂能改善呼吸道病猪的临床症状，其作用机制与降低白细胞数量，抑制PMN cAMP-PDE活性和降低血浆IL-6、TNF-α含量相关。

Study on Cyclic AMP Phosphodiesterase in Polymorphonuclear Neutrophils and cAMP,IL-6,TNF-α in Plasma of Porcine Respiratory Disease Complex and the Therapeutic Effect of Chinese Herbal Medicine

The study observed the variety of cyclic AMP phosphodiesterase (cAMP-PDE) activity in polymorphonuclear neutrophils (PMN) and contents of cAMP,IL-6,TNF-α in plasma of porcine respiratory disease complex (PRDC),the therapeutic effect and influence of self-made Chinese herbal decoction,for accumulating information of respiratory inflammation pathological mechanism and its prevention.Divided pigs with PRDC into disease group and treatment group, and normal ones as control group. Drenched treatment group with 1 g crude drug/mL Chinese herbal decoction, which was composed of reed rhizome,mongolian milkvetch root,white peony root and liquorice root,twice a day and 1.5 mL/kg body weight for ten days. Observed changes of the number of white blood cell in peripheral blood,neutrophils were isolated from whole blood,HPLC was used to test its cAMP-PDE activity and ELISA kit was used to test the contents of IL-6,TNF-α in plasma.Compared with control group,the number of white blood cell in disease group increased significantly (P<0.05),cAMP-PDE activity and contents of TNF-α,IL-6 increased extremely significantly (P<0.01);but TNF-α in treatment group increased significantly (P<0.05). Compared with the disease group,the number of white blood cell,cAMP-PDE activity and content of TNF-α in treatment group decreased significantly (P<0.05), content of IL-6 decreased extremely significantly (P<0.01).The results showed that abnormal inflammatory reaction existed in PRDC,cAMP-PDE activity,the contents of IL-6 and TNF-α,the number of white blood cell increased significantly.Self-made Chinese herbal decoction could improve the clinical symptoms of the disease,of which the function mechanism was related to the reduction of the white blood cell number,inhibition of cAMP-PDE activity,and reduction of contents of IL-6 and TNF-α in plasma.