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Effect of β2-adrenergic receptor expression on migration of early-EPCs in COPD patients
Authors:LIU Xiao-ran  HUANG Xin-yan  LIN Geng-peng  TAN Wei-ping  LIU Yang-li  XIE Can-mao
Institution:1.Emergency Department, The Affiliated Hospital of Hainan Medical College, Haikou 570102, China;2.Respiratory Department, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China. E-mail: canmaoxie@aliyun.com
Abstract:AIM:To investigate whether early endothelial progenitor cells (early-EPCs) express β2-adrenergic receptor (β2AR) in the chronic obstructive pulmonary disease (COPD) patients and the effect of β2AR expression on the migration of early-EPCs. METHODS:Venous blood samples (20 mL) were obtained from antecubital vein of COPD patients or healthy controls. Peripheral blood mononuclear cells were isolated by standard Ficoll gradient centrifugation, and purified by CD34 positive selection cocktail. The mRNA expression of β2AR in the early-EPCs was detected by RT-PCR. The protein levels of β2AR were assessed by Western blotting and flow cytometry. Chemotaxis was studied by Transwell assay. Cultured early-EPCs were treated with ICI118551, norpinephrine (NE) or monoclonal antibody of β2AR (mAb-β2AR) for 24 h. The number of migratory cells was counted under a light microscope. RESULTS:The level of β2AR expression in the COPD patients was higher than that in the controls. The number of migratory early-EPCs to stromal cell-derived factor 1α was significantly improved by ICI118551 compared with other COPD groups. When early-EPCs from the COPD patients or the controls were treated with different concentrations of mAb-β2AR for 24 h, the number of migratory early-EPCs from the COPD patients and the controls treated with NE at concentration of 100 nmol/L was significantly reduced. However, a marked decrease in the number of migratory early-EPCs from the COPD patients treated with NE was observed compared with control group. Before treated with ICI118551 or NE for 24 h, the early-EPCs were co-incubated with mAb-β2AR for 40 min, and the number of migratory early-EPCs was not significantly different between COPD group and control group. Genetic down-regulation of β2AR promoted the migration of early-EPCs in COPD group. CONCLUSION:The level of β2AR expression in the COPD patients is increased compared with the controls. The down-regulation of β2AR improves the migration of early-EPCs.
Keywords:Chronic obstructive pulmonary disease  Endothelial progenitor cells  β2-Adrenergic receptor  
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