禽源致病性沙门氏菌耐药表型与耐药基因的分析

为了解江苏苏中地区禽源沙门氏菌耐药现象及耐药基因的存在情况，采用K-B法对22株沙门氏菌分离株进行12种抗菌药物敏感性试验，并通过PCR方法检测了12种耐药基因的存在情况。结果显示，所有菌株对甲氧胺嘧啶、阿米卡星、庆大霉素、萘啶酮酸、诺氟沙星的耐药率均在72.73%～100%之间；存在着5种耐药表型，每个耐药表型均在4重或4重以上，耐药最多菌株可耐受10种药物；扩增出9种耐药基因，与GenBank中的参考序列同源性均在98%以上；耐药表型与耐药基因的存在基本一致，但无绝对相关性。本研究结果表明苏中地区沙门氏菌耐药现象非常严重，且耐药基因广泛存在。

Analysis of Resistant Phenotypes and Resistant Genes of Pathogenic Salmonella from Avian

To investigate the presence of antibiotic resistance and resistant genes about avian Salmonella in Suzhong area, 22 isolates were evaluated for antimicrobial sensitivity by K-B disc diffusion method against 12 antimicrobial drug,and then 12 resistant genes of the isolates were detected by PCR. The resistant rates of all isolates were found from 72.73% to 100% to amikacin, norfloxacin and so on; 5 resistant phenotypes were detected, and each isolates were resistant to more than 4 of the 12 antimicrobial drugs,even 10 drugs; 9 different resistant genes were amplified, the homologies were very high compared with the corresponding genes in GenBank (≥98%); the resistant phenotypes and their resistance related genes were essential consistent, but no absolute correlation. The above results showed that the antibiotic resistance of Salmonella was very serious, and the resistant genes existed widely.