Effects of furin inhibitor on metastasis of human breast cancer MCF-7 cells |
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Authors: | REN Jing-li SHI Qi SUN Ming-zhen SONG Guo-hua MA Yong-chao |
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Affiliation: | 1.Key Laboratory of Medical Bioengineering, 2Department of Pharmacology,Faculty of Pharmacy, 4Department of Biochemistry, Luohe Medical college, Luohe 462000, China;3.Department of Pathology, Xinxiang Medical University, Xinxiang 453000, China. |
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Abstract: | AIM:To investigate the mechanism underlying breast cancer metastasis and to provide theoretical data for studying the pathogenesis of breast cancer onset and development. METHODS:Human breast cancer MCF-7 cells were treated with different concentrations of furin inhibitor α1-PDX for 48 h. Wound healing assay and Transwell assay were applied to detect the migration and invasion abilities of the MCF-7 cells. The expression of cell migration-associated proteins, including membrane-type 1 matrix metalloproteinase (MT1-MMP), vascular endothelial growth factor (VEGF)-C and VEGF-D, was determined by Western blotting. The protein levels of MMP2 and MMP9 in the supernatant were measured by ELISA. RESULTS:Compared with control group, 200 nmol/L of furin inhibitor exerted significant inhibitory effects on the cell migration (P<0.05). The expression of cell migration-associated proteins MT1-MMP, VEGF-C and VEGF-D was significantly inhibited after treated with α1-PDX (P<0.05). Significant inhibitory effects of α1-PDX on the expression of MMP9 and MMP2 (P<0.05) in the supernatant were observed. CONCLUSION:Furin inhibitor suppresses the metastasis of MCF-7 cells via down-regulating the expression of MMPs and VEGFs. |
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Keywords: | Furin inhibitors Breast neoplasms Matrix metalloproteinases Vascular endothelial growth factors |
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