Preliminary mechanism of senegenin against H/R-induced apoptosis of primary cortical neurons |
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Authors: | ZHAO Yan-dong LIU Pan-hong LI Xue-min LU Fan WANG Hua-dong LU Da-xiang QI Ren-bin |
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Affiliation: | 1.Department of Pathophysiology, 2Key Laboratory of Pathophysiology, State Administration of Traditional Chinese Medicine, School of Medicine, 3Department of Orthopaedics, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China. E-mail: tqirb@jnu.edu.cn |
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Abstract: | AIM:To explore the preliminary mechanism of senegenin (Sen) on inhibiting hypoxia/reoxygenation(H/R)-induced apoptosis of primary cortical neurons. METHODS:The cultured cortical neurons were randomly divided into normal group (control group), model group (H/R group), Sen+H/R group and Sen group. Flow cytometry was used to evaluate the effect of Sen on H/R-induced cell apoptosis. The protein levels of JNK, p-JNK, c-Jun, p-c-Jun, Bcl-2 and Bax were assessed by Western blotting. RESULTS:The apoptotic rate in H/R group was obviously higher than that in control group (P<0.05), while the apoptotic rate in Sen+H/R group was obviously lower than that in H/R group (P<0.05), suggesting that the model of apoptosis was established successfully. The results of Western blotting showed that Sen increased the expression of JNK and c-Jun, inhibited the phosphorylation of JNK and c-Jun (P<0.05), increased the protein level of Bcl-2 and inhibited the protein level of Bax in H/R treated primary cortical neurons (P<0.05). CONCLUSION:Sen has a protective effect against H/R-induced neuronal apoptosis by increasing the expression of JNK and c-Jun, inhibiting the phosphorylation of JNK and c-Jun, increasing the protein level of Bcl-2 and decreasing the protein level of Bax. |
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Keywords: | Senegenin Neurons Hypoxia/reoxygenation Apoptosis |
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