Survivin-2B induces apoptosis of human breast cancer cells

AIM：To explore the role of survivin-2B in the process of tumor cell apoptosis. METHODS：The survivin-2B gene was cloned into pcDNA3.1 vector and the recombinant plasmid pcDNA3.1-survivin-2B was obtained. Human breast cancer MCF7 cells were transfected with pcDNA3.1 and pcDNA3.1-survivin-2B using Lipofectamine 2000. The cell cycle was determined by propidium iodide staining, and the apoptosis was detected by annexin V/7-AAD staining 48 h after transfection. Meanwhile, tatal RNA was extrated and multiplex polymerase chain reaction based on GenomeLab GeXP Genetic Analysis System was performed to detect the expression of 21 tumor-related genes. RESULTS：Flow cytometry analysis indicated that over-expression of survivin-2B promoted the apoptosis and cell cycle arrest of MCF7 cells. Compared with control group, totally 10 differential expressed genes were related to the over-expressed survivin-2B, among which 2 were up-regulated and 8 were down-regulated. The expression of aldehyde dehydrogenase 4 family member A1 (ALDH4A1) was 48% down-regulated, and the expression of protein regulator of cytokinesis 1 (PRC1) was 1.08 folds up-regulated. CONCLUSION：Survivin-2B induces the expression changes of some tumor-related genes, which results in the apoptosis and G2/M arrest of MCF7 cells.