Quantification of tryptase and T-cell immunoglobulin mucin 1 double-positive mast cells in different degrees of human periodontitis |
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Authors: | LI Juan YIN Xiao-ping LAN Tian L Fang-li HUANG Bo CHEN Ke HUANG Shi-guang |
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Institution: | 1.Faculty of Dentistry, School of Medicine, Jinan University, Guangzhou 510632, China;2.Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China;3.Department of Stomatology, Guangzhou Women and Children Medical Centre, Guangzhou 510623, China. |
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Abstract: | AIM:To examine the expression of T-cell immunoglobulin mucin 1 (TIM-1) on tryptase-positive mast cells (MCs) in different severities of human chronic periodontitis. METHODS:Human gingival specimens (n=92) were involved in this study, including healthy control (n=27), mild chronic periodontitis (n=34) and severe chronic periodontitis (n=31). The gingival specimens were fixed in 4% formaldehyde. Paraffin embedding and serial sectioning with hematoxylin and eosin staining were performed for histopathological examination, and double-immunofluorescence staining was conducted for identification of tryptase-TIM-1 double-positive MCs in the gingival tissues. RESULTS:Compared with the healthy controls, the densities (cells/mm2) of tryptase-TIM-1 double-positive MCs were significantly increased in both mild chronic periodontitis group (P<0.05) and severe chronic periodontitis group (P<0.01). However, compared with mild chronic periodontitis group, both the score of gingival tissue inflammation and the density of tryptase-TIM-1 double-positive MCs in the gingival tissues were significantly increased in severe periodontitis group (P<0.05). CONCLUSION:Significantly increased number of tryptase-TIM-1 double-positive MCs has the similar tendency as the severity of periodontitis inflammation in human chronic periodontitis, suggesting that tryptase-TIM-1 double-positive MCs may play an important role in human chronic periodotitis. |
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Keywords: | Chronic periodontitis Mast cells Tryptase T-cell immunoglobulin mucin 1 |
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