福清山羊和戴云山羊发情周期内生殖激素分泌规律研究

为了研究福清山羊和戴云山羊发情周期内生殖激素的分泌变化规律,采用Elisa方法检测了2个品种经产母羊整个发情周期内外周血中4种生殖激素(雌二醇、孕酮、促卵泡激素、促黄体激素)的浓度。结果表明,2个品种发情周期内血浆FSH、LH均呈现周期性波动式分泌,福清山羊在发情周期中出现了4次波峰而戴云山羊为5次。2个品种血浆E_2的分泌水平呈现明显的高、低2个分泌时期,2个分泌时期血浆E_2浓度差异达到极显著水平(P0.01),戴云山羊高分泌期的E_2浓度显著高于福清山羊(P0.05)。2个品种血浆P浓度在发情期、发情前后2~3d较低,到发情周期的第6d开始分泌量迅速增加,在发情周期第8~9d达到整个发情周期的最大峰值,极显著高于发情期血浆P浓度(P0.01),随后在较高浓度范围内呈波动分泌至发情周期第16d,之后迅速下降至发情期的低分泌水平。

Reproductive Hormone Secretion of Daiyun and Fuqing Goats in Oestrus

The secretion of reproductive hormonesin Daiyun and Fuqing goats inoestrus was studied.The secretory characteristics,such as FSH,LH,E2 and P hormone levels,during the estrousperiod were monitored using ELISA.Serum FSH and LH of the two breeds of goats fluctuated cyclically 4 peaks in Fuqing goats,and 5 peaks in Daiyun.There were one high and one low E2 secretion phases with an extremely significant difference on the hormonal concentration in serum (P＜0.01).During the hypersecretion phase,E2 in Daiyun was significantly higher than that in Fuqing goats (P＜0.05).For both breeds,their Phormones lowered during oestrus as well as 2-3 days before and after oestrus.The level increased rapidly on the 6th day of the estrous cycle;reached a peak on 8th to 9th day,and was extremely significantly greater than the average during the oestrus (P＜0.01).Thereafter until the 16th day,the secretions fluctuated in a continual high range,and eventually,decreased swiftly to alow level.