彩色马铃薯茎尖培养及病毒检测技术

为了探明彩色马铃薯茎尖培养方法,获得彩色马铃薯无毒试管苗,研究了不同质量浓度植物生长调节剂组合的培养基对21份彩色马铃薯后代品系组培苗形成的影响,并用RT-PCR方法对试管苗进行马铃薯主要病毒检测。结果表明,筛选出的组合(MS+NAA0.05mg·L~(-1)+6-BA0.1mg·L~(-1)+GA30.2mg·L~(-1)+泛酸钙0.2mg·L~(-1))可作为闽薯1号茎尖诱导分化的最佳培养基,优化的植物生长调节剂组合在不同的彩色品种茎尖分化和植株形成差异较大,试管苗经RT-PCR检测6种马铃薯病毒(PVX、PVY、PVS、PVA、PVM和PSTVd),获得了11份彩色马铃薯脱毒苗,可用于大田生产用试管苗。

Stem-tip Tissue Culture and Virus Detection on Plantlets of Color Potato

Stem-tips of potato (Solanum tuberosum L.) plants that bear colored tubers were used to produce virusfree plantlets in test tubes.Culture media with hormone combinations in varying concentrations were tested to determine their effects on 21 varieties of plantlets offspring of the potato tissue culture.Meanwhile,RT-PCR was employed to detect any viruses present in the plantlets.The optimum medium for propagating a chosen cultivar,Minshu No.1,was formulated on MS with 0.05 mg · L-1 NAA,0.1 mg · L-1 6-BA,0.2 mg · L-1 GA3 and 0.2 mg · L-1 calcium pantothenate.Different hormone combinations led to significantly different stem-tip differentiation and plantlet formation among the potato varieties.A total of 11 test tube cultivars derived from the stem-tip cultures were virus-free without the contamination of PVX,PVS,PVA,PVS,PVM or PSTVd.They could be the candidate plantlets for field planting.