| 秋石斛原球茎液体增殖培养研究 |
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| 引用本文： | 叶秀仙,林榕燕,黄敏玲,钟淮钦,林兵. 秋石斛原球茎液体增殖培养研究[J]. 福建农业学报, 2017, 0(9): 959-962. DOI: 10.19303/j.issn.1008-0384.2017.09.008 |
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| 作者姓名： | 叶秀仙 林榕燕 黄敏玲 钟淮钦 林兵 |
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| 基金项目： | 福建省科技计划项目——省属公益科研院所基本科研专项，福建省财政专项——福建省农科院科技创新团队PI项目，福建省农业科学院导师制基金项目 |
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| 摘 要： | 以秋石斛‘三亚阳光’幼芽茎尖诱导形成的原球茎为增殖培养材料,采用液体培养方式及正交设计法研究基本培养基、噻重氮苯基脲(TDZ)、椰汁和白糖4种因素对秋石斛原球茎增殖的影响,以期筛选出各因子的最佳水平,建立秋石斛‘三亚阳光’原球茎液体增殖培养技术。结果表明:各试验因素对秋石斛原球茎增殖影响的主次关系为基本培养基TDZ白糖椰汁;筛选出秋石斛PLB适宜增殖培养基配方为改良HM2+TDZ 0.5mg·L~(-1)+NAA 0.1mg·L~(-1)+椰子汁20.0mL·L~(-1)+白糖30.0g·L~(-1),增殖培养35d平均增殖系数达6.65。
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The Study on Dendrobium spp.PLB Propagation in Liquid Culture |
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| Abstract: | The protocorm-like body (PLB) induced from stem tips of Dendrobium spp.Sanya Sunny'were used as explants,The orthogonal design and the liquid media were used to study the effects of the key factors such as culture medium,TDZ,eoconut water and sugar contained on PLB propagation of Dendrobium spp.‘Sanya Sunny’.Through above research,it was tried to find the main factors and build up the PLB proliferation the system in Dendrobium spp.Sanya Sunny'in the paper.The results showed that the main factors of PLB propagation are the basic medium,TDZ,sugar and coconut water.The best multiplication medium of PLB is improvement HM # 2 medium,TDZ 0.5 mg · L-1,NAA 0.1 mg · L-1,coconut water 20.0 mL · L-1,and sugar 30.0 g L-1 to result in an averaged propagation coefficient of 6.25 in 35 days. |
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