| Rd29A启动子在小麦幼胚愈伤组织中的活性研究 |
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| 引用本文： | 高世庆,陈明,马有志,程宪国,杜丽璞,徐惠君.Rd29A启动子在小麦幼胚愈伤组织中的活性研究[J].作物学报,2005,31(2):150-153. |
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| 作者姓名： | 高世庆 陈明 马有志 程宪国 杜丽璞 徐惠君 |
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| 作者单位： | 中国农业科学院作物育种栽培研究所，农业部作物遗传育种重点开放实验室，北京100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 来自拟南芥的Rd29A基因受干旱、高盐及低温诱导表达，编码一种与LEA蛋白相似的亲水性很强的蛋白。用Rd29A启动子控制GUS报告基因的表达构建载体，通过基因枪介导转化小麦幼胚愈伤组织，经过分子生物学检测及在PEG胁迫条件下的GUS组织化学染色证实了Rd29A启动子在小麦愈伤组织中能够诱导GUS基因的表达。
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| 关 键 词： | Rd29A启动子 遗传转化 小麦 |
| 收稿时间： | 2003-12-22 |
| 修稿时间： | 2003年12月22 |
Activity of rd29A Promoter in Wheat Immature Embryonic Calli |
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| GAO Shi-Qing,CHEN Ming,MA You-Zhi,CHENG Xian-Guo,DU Li-Pu,XU Hui-Jun.Activity of rd29A Promoter in Wheat Immature Embryonic Calli[J].Acta Agronomica Sinica,2005,31(2):150-153. |
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| Authors: | GAO Shi-Qing CHEN Ming MA You-Zhi CHENG Xian-Guo DU Li-Pu XU Hui-Jun |
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| Institution: | Key Laboratory of Crop Genetics and Breeding, Ministry of Agricultural/ Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081 |
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| Abstract: | rd29A gene from Arabidopsis, encoding LEA-like protein, was induced by stresses like drought, high-salt and low-temperature. An expression vector containing GUS gene under control of rd29A promoter was constructed and bombarded into wheat immature embryogenic calli by biolistic. The results of PCR, PCR-Southern and histochemical stain showed that expression vector was transferred into wheat calli and GUS gene was induced by PEG treatment. |
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| Keywords: | rd29A promoter Genetic transformation Wheat |
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