| 菊苣高效遗传转化体系的建立 |
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| 引用本文： | 程林梅,曹秋芬,黄静,孙毅,高洪文,王红宝.菊苣高效遗传转化体系的建立[J].草业学报,2004,13(6):112-116. |
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| 作者姓名： | 程林梅 曹秋芬 黄静 孙毅 高洪文 王红宝 |
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| 作者单位： | 山西省农业生物技术研究中心,山西,太原,030031;中国农业科学院草业中心,北京,100081;山西省农业科学院畜牧兽医研究所,山西,太原,030032 |
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| 基金项目： | 山西省留学回国人员科研项目
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山西省农业科学院高新技术项目 |
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| 摘 要： | 研究了影响农杆菌GV1301菌株介导的菊苣遗传转化的几种因素,建立了菊苣高效遗传转化体系,获得了转基因抗性苗.结果表明,采用菊苣叶片,经预培养和共培养2 d,农杆菌浸泡时间为5～7 min,卡那霉素20 mg/L,头孢霉素600 mg/L,最高转化频率达7.1%,经PCR检测,目的基因已整合到菊苣基因组中.
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| 关 键 词： | 菊苣 农杆菌介导 高效遗传转化 |
| 文章编号： | 1004-5759(2004)06-0112-05 |
| 修稿时间： | 2003年9月16日 |
Establishment of highly-efficient genetic trasformation system in Cichorium intybus |
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| CHENG Lin-mei,CAO Qiu-fen,HUANG Jing,SUN Yi,GAO Hong-wen,WANG Hong-bao.Establishment of highly-efficient genetic trasformation system in Cichorium intybus[J].Acta Prataculturae Sinica,2004,13(6):112-116. |
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| Authors: | CHENG Lin-mei CAO Qiu-fen HUANG Jing SUN Yi GAO Hong-wen WANG Hong-bao |
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| Institution: | CHENG Lin-mei~1,CAO Qiu-fen~1,HUANG Jing~1,SUN Yi~1,GAO Hong-wen~2,WANG Hong-bao~3 |
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| Abstract: | Factors affecting genetic transformation of Cichorium intybus by Agrobacterium tumefaciens were studied and a highly efficient system for genetic transformation of Cichorium intybus was developed. The result indicated that the optimized conditions for transforming Cichorium intybus leaf fragments were pre-culturing explants for 2 d, infecting them by Agrobacterium tumofaciens for 5-8 min, co-culturing for 2 d, and then regenerating on the medium with kanamycin 20 mg/L and cefotaxime 600 mg/L. The transformation frequency was 7.1%. PCR amplification verified that the target gene had been integrated into genome of Cichorium intybus. |
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| Keywords: | Cichorium intybus Agrobacterium tumefaciens-mediation genetic transformation |
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