| 正交试验设计在优化甘蔗再生体系中的应用 |
| |
| 引用本文： | 薛志平,林美娟,陈平华,陈如凯.正交试验设计在优化甘蔗再生体系中的应用[J].江西农业大学学报,2005,27(3):389-393. |
| |
| 作者姓名： | 薛志平 林美娟 陈平华 陈如凯 |
| |
| 作者单位： | 福建农林大学,农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350002 |
| |
| 基金项目： | 国家863计划(2001AA241191,2002AA241031)，福建省青年科技人才创新项目(2001J044)，948引进国际先进技术项目(2003-Q06) |
| |
| 摘 要： | 建立高效再生体系是生产甘蔗健康种苗和进行遗传改良的前提。试验采用正交设计方法，研究了6-BA、KT、NAA、活性炭的不同浓度及其组合对两个甘蔗基因型愈伤组织分化的效果，并筛选出了优化处理组合。试验研究结果表明，对于福农95-1702和福农94-0403的愈伤组织分化效果最佳的组合分别是：MS 1.5mg／L6-BA 2．0mg／LKT 0．2mg／LNAA 1．0g／L活性炭和MS 2．5mg／L6-BA 1．5mg／LKT 1，0g／L活性炭。
|
| 关 键 词： | 甘蔗愈伤组织 再生体系 优化 正交设计 |
| 文章编号： | 1000-2286(2005)03-0389-05 |
| 修稿时间： | 2005年1月13日 |
Application of Orthogonal Design in Experiments on Optimum Regeneration System for Sugarcane |
| |
| XUE Zhi-ping,LIN Mei-juan,CHEN Ping-hua,CHEN Ru-kai.Application of Orthogonal Design in Experiments on Optimum Regeneration System for Sugarcane[J].Acta Agriculturae Universitis Jiangxiensis,2005,27(3):389-393. |
| |
| Authors: | XUE Zhi-ping LIN Mei-juan CHEN Ping-hua CHEN Ru-kai |
| |
| Institution: | XUE Zhi-ping,LIN Mei-juan,CHEN Ping-hua,CHEN Ru-kai~* |
| |
| Abstract: | Establishing high efficiency regeneration systems were precondition of yielding healthy seedling and genetic improvement for sugarcane. The effects of different concentration and combinations of 6-BA, KT, NAA and active carbon on two genotype sugarcane callus for their regeneration were studied by using orthogonal design, and combinations of optimum treatment were selected. The results showed that the optimum combinations were MS+1.5 mg/L 6-BA+2.0 mg/L KT+0.2 mg/L NAA+1.0 g/L active carbon and MS+2.5 mg/L 6-BA+1.5 mg/L KT+1.0 g/L active carbon for FN95-1702 and FN94-0403 respectively. |
| |
| Keywords: | sugarcane callus regeneration system optimum orthogonal design |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
