| 珍稀濒危鸡种不同取样方法DNA提取的研究 |
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| 引用本文： | 李鹏,刘文忠,栗艳,武建亮.珍稀濒危鸡种不同取样方法DNA提取的研究[J].山西农业大学学报(自然科学版),2009,29(4):298-302. |
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| 作者姓名： | 李鹏 刘文忠 栗艳 武建亮 |
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| 作者单位： | 山西农业大学,动物科技学院,山西,太谷,030801 |
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| 基金项目： | 教育部"新世纪优秀人才支持计划"资助项目 |
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| 摘 要： | 为了克服珍稀濒危鸡种在分子生物学研究中存在的取样局限性,拓宽取样范围,对鸡的血液、精液、羽毛和死胎蛋的DNA提取进行了研究。结果表明不同取样部位所提DNA浓度均适合于生物学实验要求。该研究中有关方法的应用将为珍稀濒危鸡种的分子生物学研究提供更广阔的DNA来源。
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| 关 键 词： | 鸡 珍稀濒危品种 取样方法 DNA提取 |
Effects of Different Sampling Methods on the DNA Extraction in Rare and Endangered Chicken Breeds |
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| Abstract: | This paper studies the extraction effects of DNA from whole blood,semen,feathers and death fetal eggs in rare and endangered chicken breeds,aiming at overcoming the limitation of DNA sampling and widening the sampling range in such breeds for molecular biological research.The results showed that concentration of the extracted DNA with different sampling methods was available for biological experiments.The application of relative methods will provide more DNA sample sources of samples for molecular biology research of rare and endangered chicken breeds. |
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| Keywords: | Chicken Rare and endangered breeds Extraction methods DNA extraction |
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