两种氨酸对公牛精液冷冻效果研究

[目的]试验研究L—半胱氨酸、精氨酸对牛精液冷冻效果。[方法]基础液中分别添加不同浓度的L—半胱氨酸、精氨酸进行试验,再用L₉(3₄⁾正交表优化最佳配比,获得不同的稀释液配方,然后再进行对比试验,优选出使用效果最好的牛精液冷冻稀释液。[结果]牛精液冷冻稀释液添加0.125%、0.25%、0.375%的半胱氨酸；牛精液冷冻稀释液添加0.5%、0.75%精氨酸,牛冷冻精液解冻活力均得到提高。[结论] 结果表明,半胱氨酸、精氨酸可用于牛精液冷冻保存,能改善牛精液冷冻保存效果,对提高牛冷冻精液质量效果明显。

Study on the effect of two amino acids on semen freezing in bullsLi Wen, Yang Qiang,Quan Yuehai,Li Guiyou, Li Chunping,Li Hong,

[Objective] To study the effect of L-cysteine and arginine on bovine semen freezing. [Method] Different concentrations of L-cysteine and arginine were added to the base solution for testing, and then the optimal ratio was optimized by L9(34) orthogonal table to obtain different diluent formulas, and then the comparative test was carried out to select the best frozen dilution of bovine semen. [Result] Cysteine was added with 0.125%, 0.25% and 0.375% cysteine in the frozen dilution of bovine semen. The freezing dilution of bovine semen was added with 0.5% and 0.75% arginine, and the thawing vitality of bovine frozen semen was improved. [Conclusion] The results showed that cysteine and arginine could be used for the cryopreservation of bovine semen, which could improve the cryopreservation effect of bovine semen, and had obvious effects on improving the quality of bovine frozen semen.