山羊草谷胱甘肽S-转移酶基因家族鉴定及表达分析

谷胱甘肽S-转移酶是一种多功能蛋白酶,在植物体内参与干旱、盐、低温、重金属等多种非生物胁迫的调节;山羊草是普通小麦D染色体组的供体物种,深入挖掘山羊草中GST基因,对进一步分析六倍体小麦GST基因的功能具有重要意义。本研究利用信息生物学手段,在山羊草中共发现114条GST基因序列,分属于6个亚族;基因复制分析发现共4对基因发生了基因复制,且均为纯化选择;采用荧光定量PCR对部分GST基因在非生物胁迫下的表达分析发现,8个GST基因在响应干旱和盐胁迫时,主要在根部显著上调表达,3个GST基因在响应低温胁迫时,在根和叶中均显著上调表达,说明山羊草中的GST基因在应答非生物胁迫时,在不同组织中的表达存在着差异。

Identification and Expression Analysis of the Whole Glutathione S-Transferase Genome Family in Aegilops tauschii under Abiotic Stress

Glutathione S-transferase (GST) is a multifunctional protease which involves in the regulation process of many abiotic stresses (drought, salt, low temperature, heavy metals) in plants. Aegilops tanschii is the donor of D genome for hexaploid wheat (Triticum aestivum, AABBDD), and the study on GST genome family in Aegilops tanschii (DD) will facilitate the further study of GST in hexaploid wheat. In this study, 114 GST genes from Aegilops tanschii were selected and classified into 6 subfamilies. Gene duplication analysis found that four pairs GST genes had been duplicated and they belonged to purify selection. The expression levels of some GST genes under environmental stress found that eight GST genes were highly expressed in root under drought and salt stress by qRT-PCR assay, in which three GST genes were up-regulated significantly responding to low temperature in root and leaf. This suggested there was a difference in the expression of GSTs in Aegilops tauschii responding to abiotic stress in root and leaf.