利用SSR标记和产量对27份玉米自交系进行杂种优势群划分

利用SSR标记技术对23份玉米自交系和4个测验种进行杂优类群研究。从117对引物中筛选出77对扩增带谱清晰且具有多态性的SSR引物，在供试材料中检测到398个等位基因变异，计算27个自交系间的遗传距离(GD)在0.107 4～0.438 0，平均0.288 0。分析GD和产量发现，GD大于0.310 0的组合具有明显产量优势，GD小于0.288 0组合的产量优势较弱；根据分子聚群并结合产量和系谱来源分析，将27份自交系划分为3个杂种优势群A群、B群和C群。

Heterotic Grouping of 27 Maize Inbred Lines Divided by SSR Markers and Yields

Heterotic grouping of 23 maize inbred lines and 4 testers were classified by SSR markers. A set of 77 polymorphic SSR primers were selected from 117 primers, Genetic distances(GD) based on SSR markers of 27 lines ranging from 0.107 4 to 0.438 0, with an average of 0.288 0. Comparison of GD with yields, it could be found that the combinations with GD > 0.310 0 had great yield heterosis, while the combinations with GD < 0.288 0 had low yield heterosis. Combined with molecular markers and yield as well as pedigree data, the 27 inbred lines were classified into three heterotic groups A, B and C.