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| 卵泡液对不同大小牛腔前卵泡体外培养过程中E2分泌的影响 | |
| 引用本文： | 高志花,刘立文,付永斌,程玉芳,王净,周虚.卵泡液对不同大小牛腔前卵泡体外培养过程中E2分泌的影响[J].西北农业学报,2007,16(5):18-21. |
| 作者姓名： | 高志花 刘立文 付永斌 程玉芳 王净 周虚 |
| 作者单位： | 1. 河北北方学院牧工系,河北张家口,075131;吉林大学畜牧兽医学院,吉林长春,130062 2. 河北北方学院牧工系,河北张家口,075131 3. 张家口农业科学研究所,河北宣化,075131 4. 吉林大学畜牧兽医学院,吉林长春,130062 |
| 基金项目： | 吉林省杰出青年科学基金;张家口市科技局应用基础研究项目 |
| 摘 要： | Φ60~150μm牛腔前卵泡在有、无促性腺激素存在下,分别添加0、10%、15%、20%牛卵泡液(BFF)的McCoy′s 5 a无血清系统体外培养15 d,测定其雌二醇(E2)分泌量。结果显示:各添加卵泡液组在培养3、6、9、12 d的E2分泌量均显著高于对照组(0组)(P<0.01)。当培养液中有促性腺激素(FSH、LH)存在下,各添加卵泡液组的E2分泌量更高,其中添加10%牛卵泡液组中Φ>100μm腔前卵泡在培养6 d时E2分泌量达最高,为48.96±11.54 pg/mL。表明培养液中添加一定比例牛卵泡液(BFF)可显著刺激体外培养腔前卵泡E2的分泌,促性腺激素存在下更加强了卵泡液对E2分泌的刺激作用。 |
| 关 键 词： | 牛卵泡液 腔前卵泡 体外培养 雌二醇(E2) |
| 文章编号： | 1004-1389（2007）05-0018-04 |
| 收稿时间： | 2006-12-21 |
| 修稿时间： | 2007-04-05 |
The Effects of Bovine Follicular Fluid(BFF) on E2 Secretion of Different Sizes Bovine preantral Follicles during in Vitro Culture |
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| GAO Zhi-hu,LIU Li-wen,FU Yong-bin,CHENG Yu-fang and WANG Jing.The Effects of Bovine Follicular Fluid(BFF) on E2 Secretion of Different Sizes Bovine preantral Follicles during in Vitro Culture[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(5):18-21. | |
| Authors: | GAO Zhi-hu LIU Li-wen FU Yong-bin CHENG Yu-fang and WANG Jing |
| Institution: | Faculty of Animal Science and Technology, Hebei North University, Zhangjiakou Hebei 075131, China;College of Animal Science and Veterinary Medicine, Jilin University, Changchun Jilin 130062, China;Faculty of Animal Science and Technology, Hebei North University, Zhangjiakou Hebei 075131, China;Agricultural Institute of Zhangjiakou, Xuanhua Hebei 075131, China;Faculty of Animal Science and Technology, Hebei North University, Zhangjiakou Hebei 075131, China;Faculty of Animal Science and Technology, Hebei North University, Zhangjiakou Hebei 075131, China;College of Animal Science and Veterinary Medicine, Jilin University, Changchun Jilin 130062, China |
| Abstract: | Bovine preantral follicles(about 60~150 min diameter) were cultured in vitro in the McCoys 5a serum-free system supplementing with different doses of bovine follicular fluid(BFF)in presence or absence of gonadotrophins for 15 days.Estradiol(E2) production quantity were determined every 3rd day.Results demonstrated that E2 secretion quantity in each different doses of BFF group was remarkably higher than the comparison group(0 group) in the 3rd,6th,9th and 12th day of culture separately,and it became more high in the presence of gonadot rophins(FSH and LH).The level of oestradiol(E2)arrived the hig hest value(48.24 pg/mL) in the sixth day of culture for follicles with a diameter of 100 m above in the 10% BFF group.These results revealed that the addition of BFF can significiantly promote E2 production of preantral follicles in vitroculture,which was augmented in the presence of gonadotrophins. |
| Keywords: | Bovine follicular fluid(BFF) Preantral follicles Culture in vitro Estradiol(E2) |
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