| 松材线虫纤维素酶的分离纯化及性质研究 |
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| 引用本文： | 张锴,梁军,张星耀. 松材线虫纤维素酶的分离纯化及性质研究[J]. 林业科技, 2010, 35(3): 22-26 |
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| 作者姓名： | 张锴 梁军 张星耀 |
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| 作者单位： | 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京,100091 |
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| 基金项目： | 国家"十一五"科技支撑项目，科技部国际合作项目 |
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| 摘 要： | 以浙江省松材线虫病疫区马尾松样品中分离得到的松材线虫为研究对象，对其分泌物中纤维素酶的活力和酶学性质进行分析，并与试验筛选出的伴生真菌所分泌的纤维素酶进行对比。试验结果证明，两者在酶促反应最适温度、酶促反应最适pH值、金属离子对酶活力影响方面均有明显差异。
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| 关 键 词： | 松材线虫 纤维素酶 活性 酶学性质 |
Study on Purification and Enzyme Properties of Cellulase Excreted by Bursaphelenchus xylophilus |
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| Affiliation: | ZHANG Kai (Research Institute of Forest Ecology, Environmental and Protection, Chinese Academy of Forestry, Beijing 100091 ) |
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| Abstract: | Enzyme activities and character of secretion cellulase of Bursaphelenchus xylophilus and associated fungus that isolated from masson pine in Zhejiang province were analyzed. The results showed that cellulase excreted by B. xylophilus has specificity not only on enzyme activity, but also on characters to associated fungus. |
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| Keywords: | Bursaphelenchus xylophilus CeUulase Enzyme activity Enzyme character |
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