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长白山地区主要槭属植物遗传亲缘关系研究
引用本文:刘旭,郭太君,王刚.长白山地区主要槭属植物遗传亲缘关系研究[J].安徽农业科学,2010,38(20):10580-10581,10583.
作者姓名:刘旭  郭太君  王刚
作者单位:吉林农业大学园艺学院,吉林长春,130118;吉林省白河林业局两江林场,吉林白山,133600
摘    要:目的]为槭属植物的分类与利用提供科学依据。方法]利用ISSR标记对长白山地区9种主要槭属植物进行遗传多样性分析。结果]从58条引物中筛选出37条,用于东北地区槭属植物的ISSR分析,共扩增出632条带,其中多态性带604条,多态性为95.57%。将9份材料分为4类:第1类包括白牛槭、拧筋槭;第2类包括花楷槭、簇毛槭;第3类包括糖槭、色木槭、元宝槭;第4类包括茶条槭、假色槭。结论]长白山地区主要槭属植物ISSR标记结果与叶片形态学有一定的相似性,但多数试材的亲缘关系与形态特征关系不大。

关 键 词:槭属植物  ISSR  聚类分析  遗传多样性

The Study on the Relationship of Major Acer Linn in Changbai Mountain Area of China
Institution:LIU Xu et al(College of Horticulture,Jilin Agricultural University,Changchun,Jinlin 130118)
Abstract:Objective] The aim was to provide the scientific basis for Acer Linn 's classification and the resources utilization.Method] Nine major Acer Linn.in Changbai Mountain area of China were used as materials for analyzing their genome polymorphism by ISSR marker.Result] 37 pimers selected from 58 primer were used for ISSR amplification.A total of 632 bands were generated,of which 604 bands were polymorphic bands(the percentage of polymorphic band,PPB=95.57%).the 9 Acer Linn.were divided into 4 groups.the first group contented A.mandshuricum Maxim.and A.triflorum kom;The second group contented A.ukurunduense Trautv.et Mey.and A.barbinerve Maxim.;The third group contented A.Negundo Linn.,A.mono Maxim.and A.truncatum Bunge.The fourth group contented A.ginnala Maxim.and A.pseudo-sieboldianum(Pax)Kom.Conclusion] From the clustering result,The classification system of Acer Linn.in Changbai Mountain based on leves had a little significant correlation,there was no obvious difference in Acer Linn.among the clustering result.
Keywords:ISSR
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