Localization of potato leafroll virus in leaves of secondarily-infected potato plants |
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| Authors: | J F J M van den Heuvel C M de Blank D Peters J W M van Lent |
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| Institution: | (1) Department of Virology, Wageningen Agricultural University, P.O. Box 8045, 6700 EM Wageningen, The Netherlands;(2) DLO Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW Wageningen, The Netherlands |
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| Abstract: | Potato leafroll virus (PLRV) antigen was localized by immunogold labelling in semi-thin leaf sections of secondarily-infected potato plants cv. Bintje. Viral antigen was present in all cell types of the phloem tissue. but occurred most abundantly in the companion cells. Detectable amounts of PLRV antigen were found only in the sieve elements in veins with a large number of infected companion cells. Occasionally, parenchyma cells were also found to be infected. PLRV was not exclusively limited to the phloem tissue in the infected potato plants, but was also found in mesophyll cells neighbouring minor phloem vessles. Spread of virus from cell to cell in the mesophyll was not observed. The distribution of PLRV in the potato leaf tissue has implication on its availability, for acquisition by aphids. |
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| Keywords: | epipolarization microscopy immunogold labelling luteovirus monoclonal antibodies silver enhancement virus transmission |
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