中国对虾1种球形病毒及肝胰腺病变的电镜观察↑（*）

在１９９４年山东地区对虾暴发性流行病研究中，在未检出杆状病毒的中国对虾样品中首次发现１种球形病毒，电镜下观察到该病毒外有囊膜，直径２００ｎｍ左右，在细胞质中形成球形包涵体，以宿主肝胰腺和心脏为主要靶器官；受其感染的肝胰腺细胞的细胞器出现一系列病理变化：线粒体从增生、肿胀、内嵴溶解到出现纤维化，最后崩解；糙面内质网也明显增生、脱颗粒、排列紊乱，严重感染时，肿胀成泡状，甚至分解；细胞核出现病变较晚且较轻。人工感染实验证明该病毒可引起中国对虾暴发性流行病的症状，１３ｄ累积死亡率可达９０％。

Electron microscopic observation of spherical virus and pathological change of hepatopancreas in Penaeus chinesis

With electron microscope,a kind of spherical virus was observed in farmed epizootic diseased Penaeus Chinesis,but no rod-shaped virus was found.The virus is covered with envelope whose diameter is about 200 nm, and contains spherical inclusion body in cytoplasm, mainly infecting hepatopancras and hearts of its hosts.The infected epithelial cells of hepatopencreas showed a series of pathological changes in organoid that the mitochondria became hyperplastic and swollen, cristae disappearing and fibrillate up to decomposed.Meanwhile,the endoplasmic reticulum became hyperplastic obviously, granule-lost,disordered,even bubble-shaped up to decomposed when infected badly.The changes in nucleus appeared later but less seriously.Artificial infection shows that this virus can cause a sudden epizootic to shrimps with an accumulative mortality of 90% within 13 d.