井上蛀果斑螟昼夜行为节律研究

为明确井上蛀果斑螟(Assara inouei)昼夜行为节律,在温度(25±1)℃、光周期L∥D=15h∥9h、RH 60%±10%条件下,系统观察了井上蛀果斑螟的羽化、交配、产卵、卵孵化及幼虫化蛹等行为节律。结果表明:井上蛀果斑螟的羽化、交配、产卵、孵化、化蛹行为具有明显的昼夜节律。成虫的羽化主要发生在18:00-04:00,此时成虫的羽化数量占总羽化数量的80%以上,其中以20:00-22:00羽化率最高;成虫交配活动主要发生在04:00-08:00,交配率超过75%,以06:00-08:00交配率最高;单次交配持续时间为20~60min,平均为42.12min;其产卵活动主要发生在02:00-10:00,该时段所产卵量达到总产卵量的73.99%,显著高于其他时段;卵孵化主要发生在04:00-10:00和16:00-20:00两个时段,卵的孵化数量占孵化总数的82.86%,显著高于其他时段;幼虫化蛹主要发生在10:00-14:00和18:00-22:00两个时段,幼虫化蛹数占总化蛹数的72.83%,化蛹高峰为18:00-20:00。

Circadian rhythmic behavior of Assara inouei

In order to determine the circadian rhythm of Assara inouei ,the eclosion,mating,oviposition,hatch-ing and pupation were studied under the photoperiod of L∥D=15 h∥9 h at (25±1)℃ and RH of 60% ± 10% in the laboratory.The results showed that the eclosion,mating,oviposition,hatching and pupation of A.inouei had a significant biological rhythm.The adult emergence occurred mainly (80%)in 18:00-04:00,and the high-est emergence rate occurred in 20:00-22:00;75% of mating occurred in 04:00-08:00,with the highest mating rate occurring in 06:00-08:00,and the single adult mating duration was between 20-60 min,with a mean value of 42.12 min;73.99% female adults oviposited in 02:00-10:00,significantly higher than in the other periods;82.86% of egg hatching occurred in 04:00-10:00 and 16:00-20:00,significantly higher than in the other peri-ods;72.83% of the larvae pupated in 10:00-14:00 and 18:00-22:00,with the pupation peak occurring in 18:00-20:00.